-Detailed Information [JCRB1045]-

JCRB1045 OVMANA

Cell information

Important Notice(s)

JCRB1045 OVMANA: weak to freeze-thawing.

[JCRB1045 OVMANA]

OVMANA cell line is weak to freeze-thawing as the nature of this cell line. Many floating dead cells will be observed on the next day after thawing.

Unfortunately, the cell bank did not found the way to improve the viability after freeze-thawing. Once attached, the viable cells will grow with doubling time 2-3 days. The growth tends to depend on the cell density. If the growth is poor, please try to replate the cells at higher cell density.


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JCRB No.	JCRB1045	Cell Name	OVMANA
Profile	Human cell line derived from ovarian tumour.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	51
Identity	available	Tissue for Primary Cancer	ovary
Case history	ovarian carcinoma, stage IV, CDDP-IP(3) treatment done previously.	Metastasis	No
Tissue Metastasized		Genetics	ER(-), PR(-) / tumour markers, CA125, SLX, TPA(o).
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Cells are transplantable to mouse hypodermis but not to abdominal.
Classify	tumour	Established by	Gorai,I. et.al.
Registered by	Gorai,I.	Regulation for Distribution
Comment	Td=26.1 hours	Year	2002
Medium	RPMI1640 medium with 10% fetal bovine serum	Methods for Passages	Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage	5 x 10^4 cells/ml	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	ovary
Tissue Type	clear cell adeno carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3111

Reference
Pubmed id:11121861	Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells. Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139	Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20. Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723	Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties. Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618	Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice. Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M Clin Exp Metastasis. 1995 Mar;13(2):89-96

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LOT Information

09042012
04082003
02042008
05022017
10022018
08102021
10262021
10182023

Cell No.	JCRB1045	Cell Name	OVMANA
LOT No.	09042012	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	approx. 3 x 10^4 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	Slow (approx. 55-60 hrs.)	Cell Number in Vial (cells/1ml)	8.5 x 10^5
Viability at cell freezing (%)	75.5	Antibiotics Used	free
Passage Number	P60	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:13 D13S317:8,9 D7S820:10,11 D16S539:10 VWA:15,16 TH01:7,9 AM:X TPOX:8 CSF1PO:12,13
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1045	Cell Name	OVMANA
LOT No.	04082003	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal calf serum (Intergen RB52305).	Temperature	37 C
Cell Density at Seeding	1.1 x 10^4 cells/sq.cm.	Methods for Passages	Cells are harvested after treatment with 0.05% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.3 x 10^6
Viability at cell freezing (%)	83.8	Antibiotics Used	free
Passage Number	P51	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(type B), NP, LDH examined. Human not HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium containing 5% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB1045	Cell Name
LOT No.	02042008	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). The vial label (OMAMA) is wrong. Correct cell line name is OVMANA).	Temperature	37 C
Cell Density at Seeding	8 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA for 5 - 10 min at 37 C
Doubling Time	50 - 60 hrs	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	90	Antibiotics Used	free
Passage Number	P56	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1045	Cell Name	OVMANA
LOT No.	05022017	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat.# 172012)	Temperature	37 C
Cell Density at Seeding	1.3 - 6.0 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 38 hrs.	Cell Number in Vial (cells/1ml)	2.2 x 10^6
Viability at cell freezing (%)	85.0	Antibiotics Used	free
Passage Number	P58	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO (<--important), 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	85.0	Additional information

Cell No.	JCRB1045	Cell Name	OVMANA
LOT No.	10022018	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	2.9 - 4.7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time	approx. 51 hrs.	Cell Number in Vial (cells/1ml)	2.0 x 10^6
Viability at cell freezing (%)	98.4	Antibiotics Used	free
Passage Number	P54	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	80.0	Additional information

Cell No.	JCRB1045	Cell Name	OVMANA
LOT No.	08102021	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	6.3 - 8.5 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 61 hrs.	Cell Number in Vial (cells/1ml)	1.3 x 10^6
Viability at cell freezing (%)	98.1	Antibiotics Used	free
Passage Number	P57	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	99.2	Additional information

Cell No.	JCRB1045	Cell Name	OVMANA
LOT No.	10262021	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	7.7 - 8.8 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time	44 - 171 hrs.	Cell Number in Vial (cells/1ml)	8.3 x 10^5
Viability at cell freezing (%)	96.7	Antibiotics Used	free
Passage Number	P54	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	88.4	Additional information

Cell No.	JCRB1045	Cell Name	OVMANA
LOT No.	10182023	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	3.9 - 7.3 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.05% trypsin.
Doubling Time	approx. 69 hrs.	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	99	Antibiotics Used	free
Passage Number	P55	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:13 D13S317:8,9 D7S820:10,11 D16S539:10 VWA:15,16 TH01:7,9 AM:X TPOX:8 CSF1PO:12,13
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	92	Additional information