JCRB1069 NOMO-1/ADM
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1069 | Cell Name | NOMO-1/ADM |
|---|---|---|---|
| Profile | leukemia, acute monocytic, adriamycin-resistant derivative of NOMO-1 | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 31 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | acute monocytic leukemia | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Adriamycin-registant derived from NOMO-1 (IFO50474). |
| Classify | Established by | ||
| Registered by | Regulation for Distribution | ||
| Comment | Year | 2003 | |
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | ||
| CO2 Conc. | Tissue Sampling | peripheral blood | |
| Tissue Type | Promonocytic leukemia cells |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1845845 | FDP D-dimer induces the secretion of interleukin-1, urokinase-type plasminogen activator, and plasminogen activator inhibitor-2 in a human promonocytic leukemia cell line. Hamaguchi M,Morishita Y,Takahashi I,Ogura M,Takamatsu J,Saito H Blood. 1991 Jan 1;77(1):94-100 |
| Pubmed id:1701357 | Up-regulation of transferrin receptor gene expression by granulocyte colony-stimulating factor in human myeloid leukemia cells. Morishita Y,Kataoka T,Towatari M,Ito T,Inoue H,Ogura M,Morishima Y,Saito H Cancer Res. 1990 Dec 15;50(24):7955-61 |
| Pubmed id:1699657 | Enhanced expression of DNA topoisomerase II by recombinant human granulocyte colony-stimulating factor in human leukemia cells. Towatari M,Ito Y,Morishita Y,Tanimoto M,Kawashima K,Morishima Y,Andoh T,Saito H Cancer Res. 1990 Nov 15;50(22):7198-202 |
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LOT Information
| Cell No. | JCRB1069 | Cell Name | |
|---|---|---|---|
| LOT No. | 10032003 | Lot Specification | distribution |
| Medium | RPMI1640 medim with 10% fetal bovine serum and 300 nM adriamycin. Adriamycin should be added after the cells reaching log growth phase. | Temperature | 37 C |
| Cell Density at Seeding | 1-1.5 x 10^5 cells/ml | Methods for Passages | Dilution (a part of cells attaches to dish but they were neglected at passage. |
| Doubling Time | D.T. = ca. 1.5 days | Cell Number in Vial (cells/1ml) | 2.9 x 10^6 |
| Viability at cell freezing (%) | 85.5 | Antibiotics Used | free |
| Passage Number | P7* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD, LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 (without adriamycin) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |