-Detailed Information [JCRB1094]-

JCRB1094 SUIT-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB1094	Cell Name	SUIT-2
Profile	Human pancreatic cancer cell line establesh from liver metastasis.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	73
Identity	available	Tissue for Primary Cancer	pancreas
Case history	pancreatic cancer (entire pancreas and extended into other tissues, blood group was A).	Metastasis	Yes
Tissue Metastasized		Genetics	Mode of chromosome=45 (34-176)
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	PDL=38.2 hrs
Classify	tumor	Established by	Iwamura,T. et al.
Registered by	Iguchi,H.	Regulation for Distribution
Comment		Year	2003
Medium	Eagle's minimal essential medium with 10% fetal bovine serum was used at the establishment of cells. Current medium for prpagation is RPMI 1640 medium with 10% FBS.	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin in PBS(-). Subculture once a week.
Cell Number on Passage	1/8 - 1/32 splits.	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	metastatic liver tumor
Tissue Type	moderately differentiated tubular adenocarcin

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3172
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1240219

Reference
Pubmed id:3130361	Immunohistochemistry of human gastrointestinal cancer-associated antigen detected by monoclonal antibody PA 8-15. Yoshimura R,Sakamoto K,Egami H,Akagi M Jpn J Cancer Res. 1988 Feb;79(2):255-63
Pubmed id:2445616	Kinetics of carcinoembryonic antigen and carbohydrate antigen 19-9 production in a human pancreatic cancer cell line (SUIT-2). Iwamura T,Katsuki T Gastroenterol Jpn. 1987 Oct;22(5):640-6
Pubmed id:3102439	Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinoembryonic antigen and carbohydrate antigen 19-9. Iwamura T,Katsuki T,Ide K Jpn J Cancer Res. 1987 Jan;78(1):54-62

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LOT Information

09252012
08022010
09152009
01182007
04122021
03172023

Cell No.	JCRB1094	Cell Name
LOT No.	09252012	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	1.0 - 5.3 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	29 - 33 hrs	Cell Number in Vial (cells/1ml)	4.1 x 10^6
Viability at cell freezing (%)	89.6	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1094	Cell Name	SUIT-2
LOT No.	08022010	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911)	Temperature	37 C
Cell Density at Seeding	3.46-5.45X10^4cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	5.28x10^6
Viability at cell freezing (%)	96.6	Antibiotics Used	free
Passage Number	p7*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11,12 D13S317:9,11 D7S820:9,11 D16S539:9 VWA:16,17 TH01:6 AM:X TPOX:8,10 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

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Cell No.	JCRB1094	Cell Name
LOT No.	09152009	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	approx. 2 x 10^5 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time	20 hrs.	Cell Number in Vial (cells/1ml)	4.5 x 10^6
Viability at cell freezing (%)	91.2	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1094	Cell Name	SUIT-2
LOT No.	01182007	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS lot;GIBCO 4218333S).	Temperature	37 C
Cell Density at Seeding	1.1-1.7x10^5 cells/ml	Methods for Passages	Cells are treaed with 0.25% trypsin and 0.02% EDTA before passage. Passage twice a week.
Doubling Time	Td = 48 hrs	Cell Number in Vial (cells/1ml)	4.1 x 10^6
Viability at cell freezing (%)	97.1	Antibiotics Used	free
Passage Number	P7*	PDL	UNK
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD(typeB), MD and LD.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	Culture medium with 5% DMSO	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1094	Cell Name	SUIT-2
LOT No.	04122021	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.4 - 4.5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 38 hrs.	Cell Number in Vial (cells/1ml)	1.8 x 10^6
Viability at cell freezing (%)	99.5	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	97.8	Additional information

Cell No.	JCRB1094	Cell Name	SUIT-2
LOT No.	03172023	Lot Specification	distribution
Medium	RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012,　Lot#12J396)	Temperature	37 C
Cell Density at Seeding	3.04-3.23x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.04x10^6
Viability at cell freezing (%)	97.00	Antibiotics Used	free
Passage Number	p7*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11,12 D13S317:9,11 D7S820:9,11 D16S539:9 VWA:16,17 TH01:6 AM:X TPOX:8,10 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	94.00	Additional information

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