JCRB1142 HEC-265
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1142 | Cell Name | HEC-265 |
|---|---|---|---|
| Profile | Human endometrioid adenocarcinoma, G1 from uterus. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | |
| Identity | available | Tissue for Primary Cancer | uterus |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | endometrial carcinoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | endometrial carcinoma |
| Classify | tumor | Established by | Fujisawa,T. & Kuramoto, H. |
| Registered by | Kuramoto, H. | Regulation for Distribution | free |
| Comment | Year | 2005 | |
| Medium | Eagle's minimal essential medium with 15% fetal calf serum. | Methods for Passages | Cells were harvested after treatment with 0.1% trypisn and 0.02% EDTA. |
| Cell Number on Passage | 1-2 x10^5 cells/mL | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | uterine corpus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15171304 | Establishment and characterization of two different types of new human endometrial adenocarcinoma cell lines (HEC-251 and HEC-265). Fujisawa T,Hamano M,Hata H,Kamata Y,Watanabe J,Sekimoto R,Kuramoto H Eur J Gynaecol Oncol. 2004;25(3):299-304 |
| Pubmed id:15053063 | Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines. Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H Eur J Gynaecol Oncol. 2004;25(1):55-60 |
| Research results by users. Click！ | |
|---|---|
| Pubmed id:none |
ヒト子宮類内膜がん細胞株表面における免疫チェックポイント分子PD-L1の発現調節に果たすEzrin/Radixin/Moesinの役割 小堀 宅郎, 田中 千都, 伊藤 優衣, 浦嶋 庸子, 伊藤 卓也, 高垣 伸匡, 小畑 友紀雄 Journal of AYA Oncology Alliance Vol.3 No.1 2023 |
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LOT Information
| Cell No. | JCRB1142 | Cell Name | HEC-265 |
|---|---|---|---|
| LOT No. | 02072006 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 2.9x10^3 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.7x10^6 |
| Viability at cell freezing (%) | 91.4 | Antibiotics Used | Free |
| Passage Number | P187 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP, LDH examined. Human not HeLa |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:11,12 D7S820:10,11 D16S539:11 VWA:19,22 TH01:9 AM:X TPOX:9,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1142 | Cell Name | HEC-265 |
|---|---|---|---|
| LOT No. | 06132013 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 15% fetal calf serum. (FBS; GIBCO Cat. No. #10099) | Temperature | 37C |
| Cell Density at Seeding | 0.6-1.3 x10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypisn and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3.3 x 10^6 cells/1mL | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | P189 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB),NP,LDH were examined. Human,not HeLa |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:10,12 D13S317:11,12,13 D7S820:10,11 D16S539:11 VWA:19,21,22,23 TH01:9 AM:X TPOX:9,11 CSF1PO:11,12 |
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| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium with 10% DMSO | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94.4 | Additional information |
| Cell No. | JCRB1142 | Cell Name | HEC-265 |
|---|---|---|---|
| LOT No. | 12152017 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012). | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypisn. |
| Doubling Time | approx. 32 hrs. | Cell Number in Vial (cells/1ml) | 2.8 x 10^6 |
| Viability at cell freezing (%) | 85 | Antibiotics Used | free |
| Passage Number | P189 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 85 | Additional information |
| Cell No. | JCRB1142 | Cell Name | HEC-265 |
|---|---|---|---|
| LOT No. | 08062020 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 7 - 9 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 30 hrs. | Cell Number in Vial (cells/1ml) | 2.7 x 10^6 |
| Viability at cell freezing (%) | approx. 10 | Antibiotics Used | free |
| Passage Number | P189 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 5% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 96 | Additional information |
| Cell No. | JCRB1142 | Cell Name | HEC-265 |
|---|---|---|---|
| LOT No. | 09192023 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 107322) | Temperature | 37 C |
| Cell Density at Seeding | 1.4 - 1.7 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 25-28 hrs. | Cell Number in Vial (cells/1ml) | 2.5 x 10^6 |
| Viability at cell freezing (%) | 88.7 | Antibiotics Used | free |
| Passage Number | P190 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:10,12 D13S317:11,12 D7S820:10,11 D16S539:11 VWA:19,22 TH01:9 AM:X TPOX:9,11 CSF1PO:11,12 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 91.6 | Additional information |