JCRB1195 KMS-34

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1195 Cell Name   KMS-34
Profile   human myeloma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   60
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   Yes
Tissue Metastasized    Genetics   t(4;14)(p16.3;32.3)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   small round-cell morphology, part is multinucleate cell.
Classify   tumor Established by   Otsuki, T.
Registered by   Otsuki, T. Regulation for Distribution   
Comment    Year   2006
Medium   RPMI1640 medium with 10% featal bovine serum. Methods for Passages   Subculture twice a week by dilution(1/3).
Cell Number on Passage   50000 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   pleural effusion
Tissue Type   myeloma pleural effusion infiltration(IgAk)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2996
Reference
Pubmed id:15491288Expression of protein gene product 9.5 (PGP9.5)/ubiquitin-C-terminal hydrolase 1 (UCHL-1) in human myeloma cells.
Otsuki T,Yata K,Takata-Tomokuni A,Hyodoh F,Miura Y,Sakaguchi H,Hatayama T,Hatada S,Tsujioka T,Sato Y,Murakami H,Sadahira Y,Sugihara T
Br J Haematol. 2004 Nov;127(3):292-8
Pubmed id:11886382Interleukin 10 abolishes the growth inhibitory effects of all-trans retinoic acid on human myeloma cells.
Otsuki T,Yata K,Sakaguchi H,Kurebayashi J,Matsuo Y,Uno M,Fujii T,Eda S,Isozaki Y,Yawata Y,Yamada O,Wada H,Sugihara T,Ueki A
Br J Haematol. 2002 Mar;116(4):787-95

Images



LOT Information

Cell No.   JCRB1195 Cell Name   
LOT No.   02222010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   4.2 - 6.2 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 52 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   70.0 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1195 Cell Name   KMS-34
LOT No.   05302007 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   3.8x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.4x10^6
Viability at cell freezing (%)   80.2 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8
D7S820:10,11
D16S539:10,13,14
VWA:14
TH01:7,9
AM:X
TPOX:8,9
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1195 Cell Name   KMS-34
LOT No.   06052015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% featal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   33 - 84 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   70.0 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   70.0 Additional information   
Cell No.   JCRB1195 Cell Name   KMS-34
LOT No.   09272021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.5 - 2.0 x 10^5 cells/mL Methods for Passages   Simple dilusion (suspension culture).
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   fre
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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