JCRB1327 Dotcom
Cell information
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1327 | Cell Name | Dotcom |
|---|---|---|---|
| Profile | Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 14 week fetus |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | Gene Expression (OCT4, SOX2, NANOG, TERT): Positive, ALP: Positive, Immunostaining (OCT4, SOX2, NANOG, SSEA-4, Tra 1-60): Positive, Teratoma: Positive. |
| Classify | transformed | Established by | Umezawa, A. |
| Registered by | Umezawa, A. | Regulation for Distribution | |
| Comment | Year | 2008 | |
| Medium | human ES medium (iPSellon (cardio inc.) ) | Methods for Passages | Subculture once a week by Physical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.) |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | fetus lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:21637780 | DNA methylation dynamics in human induced pluripotent stem cells over time. Nishino K,Toyoda M,Yamazaki-Inoue M,Fukawatase Y,Chikazawa E,Sakaguchi H,Akutsu H,Umezawa A PLoS Genet. 2011 May;7(5):e1002085 |
| Research results by users. Click！ | |
|---|---|
| Pubmed id:27103433 |
Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Takayama K,Mitani S,Nagamoto Y,Sakurai F,Tachibana M,Taniguchi Y,Sekiguchi K,Mizuguchi H Biochem Biophys Res Commun. 2016 May 20;474(1):91-96 |
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LOT Information
| Cell No. | JCRB1327 | Cell Name | Dotcom |
|---|---|---|---|
| LOT No. | 09041720 | Lot Specification | seed/distribution |
| Medium | iPSellon (cardio inc.) | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | P5+17-P5+20 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | 46 | Chromosome Information | 46,XY |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium containing 10% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |