JCRB1329 Squeaky
Cell information
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1329 | Cell Name | Squeaky |
|---|---|---|---|
| Profile | Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | 14 week fetus | |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | Gene Expression (OCT4, SOX2, NANOG, TERT): Positive, ALP: Positive, Immunostaining (OCT4, SOX2, NANOG, SSEA-4, Tra 1-60): Positive, Teratoma: Negative. |
| Classify | transformed | Established by | Umezawa, A. |
| Registered by | Umezawa, A. | Regulation for Distribution | |
| Comment | Year | 2008 | |
| Medium | human ES medium (iPSellon (cardio inc.) ) | Methods for Passages | Subculture once a week by mechanical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.) |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | fetus lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:21637780 | DNA methylation dynamics in human induced pluripotent stem cells over time. Nishino K,Toyoda M,Yamazaki-Inoue M,Fukawatase Y,Chikazawa E,Sakaguchi H,Akutsu H,Umezawa A PLoS Genet. 2011 May;7(5):e1002085 |
| Images |
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LOT Information
| Cell No. | JCRB1329 | Cell Name | Squeaky |
|---|---|---|---|
| LOT No. | 09041013 | Lot Specification | seed/distribution |
| Medium | iPSellon+10ng/mL FGF-2 | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | P5+10-P5+13 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | 46 | Chromosome Information | 46,XY,der(22)t(17;22)(q21.3;p11.2) |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium containing 10% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1329 | Cell Name | Squeaky |
|---|---|---|---|
| LOT No. | 03302009 | Lot Specification | distribution |
| Medium | iPSellon | Temperature | |
| Cell Density at Seeding | Methods for Passages | Subculture once a week by mechanical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.) | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | P5+12 | PDL | |
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1329 | Cell Name | Squeaky |
|---|---|---|---|
| LOT No. | 04072009 | Lot Specification | distribution |
| Medium | Temperature | ||
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | P5+13 | PDL | NT |
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | NT | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Exoteric Gene | NT | |
| Medium for Freezing | 37 C | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |