JCRB1331 Tic
Cell information
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1331 | Cell Name | Tic |
|---|---|---|---|
| Profile | Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 14 week fetus |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Human iPS cell line derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | |
| Classify | Established by | Umezawa, A. | |
| Registered by | Umezawa, A. | Regulation for Distribution | |
| Comment | Year | 2008 | |
| Medium | human ES medium (iPSellon (cardio inc.) ) | Methods for Passages | Subculture once a week by Physical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.) |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | fetus lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:21637780 | DNA methylation dynamics in human induced pluripotent stem cells over time. Nishino K,Toyoda M,Yamazaki-Inoue M,Fukawatase Y,Chikazawa E,Sakaguchi H,Akutsu H,Umezawa A PLoS Genet. 2011 May;7(5):e1002085 |
| Research results by users. Click！ | |
|---|---|
| Pubmed id:26966071 |
Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Negoro R,Takayama K,Nagamoto Y,Sakurai F,Tachibana M,Mizuguchi H Biochem Biophys Res Commun. 2016 Apr 15;472(4):631-6 |
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| PDF File1 PDF File2 PDF File3 |
LOT Information
| Cell No. | JCRB1331 | Cell Name | Tic |
|---|---|---|---|
| LOT No. | 06082013 | Lot Specification | distribution |
| Medium | Culture medium for human iPS cells(attachmentA),FGF-2 10ng/ml | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | 0.1% Dispase(Dispase II neutral protease,grade II , Roche 04942078001 | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.8x10^4 |
| Viability at cell freezing (%) | 28.9 | Antibiotics Used | free |
| Passage Number | p21+9+4 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:11,14 D7S820:10,11 D16S539:11 VWA:15 TH01:8 AM:X,Y TPOX:8 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 10% DMSO,10ng/ml FGF-2,10uM ROCK inhibitor in culture medium for human iPS cells(attachmentB) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
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| No image |
| PDF File1 PDF File2 |