JCRB1344 delta-47

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1344 Cell Name   delta-47
Profile   Human myeloma cell line secreting IgD. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   72
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   left testicular tumor Metastasis   
Tissue Metastasized    Genetics   The cell line expresses CD4 & CD38. Aneuploid karyotype including 1q+, 2p+ & 14q+.
Life Span   infinite Crisis PDL   
Morphology   plasmacytoid Character   Cells are secreting IgD.
Classify   tumor Established by   Kubonishi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   Dilution
Cell Number on Passage   Split ratio = 1/2 - 1/3. Race   Japanese
CO2 Conc.   5% Tissue Sampling   ascites
Tissue Type   myeloma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2887
Reference
Pubmed id:1329502IgD myeloma presenting as a testicular tumor: establishment and characterization of an IgD-secreting myeloma cell line.
Ishii K,Yamato K,Kubonishi I,Taguchi H,Ohtsuki Y,Miyoshi I
Am J Hematol. 1992 Nov;41(3):218-24

Images



LOT Information

Cell No.   JCRB1344 Cell Name   
LOT No.   08022010 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.0 - 1.2 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture), split ratio = 1/2 - 1/3.
Doubling Time   approx. 50 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1344 Cell Name   delta-47
LOT No.   07212009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   4.3x10^5 cells/ml Methods for Passages   split ratio=1/2-1/3
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^6
Viability at cell freezing (%)   79.5 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:8
D7S820:10
D16S539:9,11
VWA:14,20
TH01:9
AM:X
TPOX:8,10
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1344 Cell Name   delta-47
LOT No.   10282019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37C
Cell Density at Seeding   approx. 2.1 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   81 Additional information   
CLOSE