JCRB1361 Vn1919

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1361 Cell Name   Vn1919
Profile   neuroglial and neuronal character coexpressing ependymoma cell line. Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   brain
Case history   ependymoma induced by intracerebroventricular injection of BK virus Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   GFAP (glial fibrillary acidic protein) positive, NF-L and NF-H (neurofilament) positive.
Classify   tumor Established by   Uchida, S.
Registered by   Aizawa, T. Regulation for Distribution   
Comment    Year   2009
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   0.05% trypsin, 0.002% EDTA inPBS(-)
Cell Number on Passage   split ratio = 1/8 Race   
CO2 Conc.   5% Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8538
Reference
Pubmed id:21073635Neural stem cell-like gene expression in a mouse ependymoma cell line transformed by human BK polyomavirus.
Aizawa T,Hasegawa K,Ohkumo T,Haga S,Ikeda K,Yoshikawa K
Cancer Sci. 2011 Jan;102(1):122-9
Pubmed id:221713Polyoncogenicity and insulinoma-inducing ability of BK Virus, a human Papovavirus, in Syrian golden hamsters.
Uchida S,Watanabe S,Aizawa T,Furuno A,Muto T
J Natl Cancer Inst. 1979 Jul;63(1):119-26
Pubmed id:191323Induction of papillary ependymomas and insulinomas in the Syrian golden hamster by BK virus, a human papovavirus.
Uchida S,Watanabe S,Aizawa T,Kato K,Furuno A
Gan. 1976 Dec;67(6):857-65

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LOT Information

Cell No.   JCRB1361 Cell Name   Vn1919
LOT No.   02132010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.21-2.2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.002% EDTA inPBS(-).
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.56x10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1361 Cell Name   Vn1919
LOT No.   04172018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.5-5.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.002% EDTA .
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.19x10^6
Viability at cell freezing (%)   96.14 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
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