JCRB1483 KYAE-1

Cell information

Important Notice(s)

本細胞株の民間企業への分譲について

On the MTA of this cell line with Kyoto University (for profit organizations in Japan)

本細胞株の使用について

Announcement of unavailability of this cell line to overseas researcher outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1483 Cell Name   KYAE-1
Profile   Human esophageal adenocarcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   60-year-old
Identity   available Tissue for Primary Cancer   esophagus
Case history   adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   polygonal-epithelial Character   T3M1N1 stage 4
Classify   tumor Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2011
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   Pleyral effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1825
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LOT Information

Cell No.   JCRB1483 Cell Name   KYAE-1
LOT No.   09032012 Lot Specification   distribution
Medium   RPMI-1640 and Ham's F12 medium (1:1 mixture) with 5% heat-inactivated fetal bovine serum (SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.8-5.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.25x10^6
Viability at cell freezing (%)   97.7 Antibiotics Used   
Passage Number   p12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11,12
D7S820:11,12
D16S539:11
VWA:14
TH01:6
AM:X
TPOX:8
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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