JCRB1484 JMU-RTK-2

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JCRB No.   JCRB1484 Cell Name   JMU-RTK-2
Profile   Malignant rhabdoid tumor of the kidney cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   4-month-old
Identity   available Tissue for Primary Cancer   
Case history   Malignant rhabdoid tumor of the kidney Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   polygonal-epithelial Character   highly aggressive malignancy
Classify   tumor Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   Restricted: Cell's developer's approval is required
Comment    Year   2011
Medium   DMEM medium with 10% heat-inactivated fetal bovine serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   Split ratio = 1/2 - 1/8 Race   
CO2 Conc.   5% Tissue Sampling   Kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W58
Reference
Pubmed id:19383826Identification and metastatic potential of tumor-initiating cells in malignant rhabdoid tumor of the kidney.
Yanagisawa S,Kadouchi I,Yokomori K,Hirose M,Hakozaki M,Hojo H,Maeda K,Kobayashi E,Murakami T
Clin Cancer Res. 2009 May 1;15(9):3014-22

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LOT Information

Cell No.   JCRB1484 Cell Name   JMU-RTK-2
LOT No.   08172012 Lot Specification   distribution
Medium   DMEM with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.69-2.9x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/2-1/8)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.66x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,10
D7S820:9,12
D16S539:9,11
VWA:14,18
TH01:7,10
AM:X,Y
TPOX:8,11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1484 Cell Name   JMU-RTK-2
LOT No.   11242017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   6.1 - 9.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   90.9 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.9 Additional information   
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