JCRB1493 UTA-SF2-2
Cell information
Important Notice(s)
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1493 | Cell Name | UTA-SF2-2 |
|---|---|---|---|
| Profile | UTA-SF2-2 (induced pluripotent stem cells: iPS cells) has been cultured in serum-free medium without feeders (hESF9a). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 46-year-old |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | ES-like cells derived from primary adult human dermal fibroblasts, infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | |
| Classify | transformed | Established by | Asashima, M. |
| Registered by | Asashima, M. | Regulation for Distribution | |
| Comment | Year | 2010 | |
| Medium | hESF9a | Methods for Passages | Subculture once a week by Physical method or enzyme-treatment method. Cells were maintained on bovine fibronectin in a growth factor defined serum-free medium (hESF9a) |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 10% | Tissue Sampling | dermal fibroblasts |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:21124894 | Reduction of N-glycolylneuraminic acid in human induced pluripotent stem cells generated or cultured under feeder- and serum-free defined conditions. Hayashi Y,Chan T,Warashina M,Fukuda M,Ariizumi T,Okabayashi K,Takayama N,Otsu M,Eto K,Furue MK,Michiue T,Ohnuma K,Nakauchi H,Asashima M PLoS One. 2010 Nov 23;5(11):e14099 |
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| PDF File1 PDF File2 PDF File3 |
LOT Information
| Cell No. | JCRB1493 | Cell Name | UTA-SF2-2 |
|---|---|---|---|
| LOT No. | 02212013 | Lot Specification | distribution |
| Medium | Culture medium for hESF8,FGF-2 10ng/ml,Activin A 2ng/ml(hESF9a) (attachmentA,C) | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Mechanical Passage.Split ratio 1/1-1/20 | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.9x10^5 |
| Viability at cell freezing (%) | 28.2 | Antibiotics Used | free |
| Passage Number | p27+10+11+5(p27+26) | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:12 D7S820:10,12 D16S539:12,13 VWA:15,16 TH01:6 AM:X TPOX:8 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 10% DMSO,10ng/ml FGF-2,2ng/ml Activin,5uM ROCK inhibitor in culture medium for hESF8(attachmentB) | CO2 Conc. | 10% |
| Viability immediately after thawing (%) | Additional information |
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| PDF File1 PDF File2 PDF File3 |