JCRB1525 NCI-H650-Luc
Cell information
Important Notice(s)発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines
Information for introduced genes, etc in luciferase expressing cell lines
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1525 | Cell Name | NCI-H650-Luc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line (NCI-H650; Human lung cancer cell line). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | |
| Identity | available | Tissue for Primary Cancer | |
| Case history | bronchioalveolar carcinoma; non-small cell lung cancer | Metastasis | Yes |
| Tissue Metastasized | Genetics | pMSCV-luc transfected. Luciferase stably expressing cell line. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | spherical | Character | puromycin-resistant |
| Classify | transformed | Established by | Murakami, T. |
| Registered by | Murakami, T. | Regulation for Distribution | To exchange and follow MTA between oversea end-user and cell-developer. |
| Comment | Year | 2011 | |
| Medium | DMEM:F12+ 0.02 mg/ml insulin, 0.01 mg/ml transferring, 25 nM Na2O3Se, 50 nM Hydrocortisone, 1 ng/ml EGF (do not filter), 0.01 mM ethanolamine, 0.01 mM phosphorylethanolamine, 100 pM triiodothyronine, 0.5% BSA, 0.5mM sodium pyruvate, extra 2mM L-glutamine | Methods for Passages | Simple dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | lung |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1525 | Cell Name | NCI-H650-Luc |
|---|---|---|---|
| LOT No. | 12262016 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.8-3.4x10^4 cells/sq.cm | Methods for Passages | dilution(Split ratio=1/2) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.8x10^6 |
| Viability at cell freezing (%) | 98.00 | Antibiotics Used | free |
| Passage Number | p20* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:8,12 D7S820:10,11 D16S539:8,11 VWA:17 TH01:6,9.3 AM:X TPOX:8,11 CSF1PO:10 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | The growth medium was changed to RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 10% heat inactivated fetal bovine serum from serum free medium. Ampoules were printed p14* by mistake.p20* is correct. |
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