JCRB1565 Skipper
Cell information
Important Notice(s)Distribution(lot no.03132014)は、1vialあたりの細胞数が非常に少ないため、24well plateで1wellに解凍して下さい
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1565 | Cell Name | Skipper |
|---|---|---|---|
| Profile | ES-like cells derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | 14 week fetus | |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | ES-like cells derived from human fetus lung cells (MRC-5), infected with recombinant retroviruses expressing the four factors (Oct3/4, Sox2, Klf4 and c-Myc). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | ES-like | Character | |
| Classify | transformed | Established by | Umezawa, A. |
| Registered by | Umezawa, A. | Regulation for Distribution | |
| Comment | Year | 2008 | |
| Medium | human ES medium (iPSellon (cardio inc.) ) | Methods for Passages | Subculture once a week by mechanical method or enzyme-treatment method. (Cells were maintained on mouse primary embryonic fibroblast feeder cells inactivated with mitomycin C.) |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | fetus lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:21637780 | DNA methylation dynamics in human induced pluripotent stem cells over time. Nishino K,Toyoda M,Yamazaki-Inoue M,Fukawatase Y,Chikazawa E,Sakaguchi H,Akutsu H,Umezawa A PLoS Genet. 2011 May;7(5):e1002085 |
| Images |
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| No image |
| PDF File1 PDF File2 PDF File3 |
LOT Information
| Cell No. | JCRB1565 | Cell Name | Skipper |
|---|---|---|---|
| LOT No. | 03132014 | Lot Specification | distribution |
| Medium | Culture medium for human iPS cells (attachmentA) ,FGF-2 10ng/ml | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Mechanical Passage/0.1%Dispase(Dispase II neutral protease,grade II,Roche 04942078001).(Split ratio=1/1-1/3) | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 6.5x10^4 |
| Viability at cell freezing (%) | 33.0 | Antibiotics Used | |
| Passage Number | p4+8+6(p4+14) | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:11,14 D7S820:10,11 D16S539:9,11 VWA:15 TH01:8 AM:X,Y TPOX:8 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 10% DMSO,10ng/ml FGF-2,10uM ROCK inhibitor in Culture medium for human iPS cells(attachmentB) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |