JCRB1584 TYUC-1-Luc
Cell information
Important Notice(s)Announcement of unavailability of this cell line to overseas researcher outside of Japan
発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines
Information for introduced genes, etc in luciferase expressing cell lines
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1584 | Cell Name | TYUC-1-Luc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line. Human esophageal adenocarcinoma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 56-year-old |
| Identity | available | Tissue for Primary Cancer | esophagus |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | pLVSIN-luc transfected TYUC-1 cells. Luciferase stably expressing cell line. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | floating | Character | undifferentiated carcinoma, T4M1N0 stage 3. Puromycin-resistant. |
| Classify | transformed | Established by | Murakami, T. |
| Registered by | Murakami, T. | Regulation for Distribution | not available for oversea researchers. |
| Comment | Year | 2014 | |
| Medium | Ham's F12 medium + DMEM (1 to 1 mix) with 5% fetal calf serum. | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | mouse xenograft |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
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-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:25667451 | MicroRNA profiles to predict postoperative prognosis in patients with small cell carcinoma of the esophagus. Okumura T,Shimada Y,Omura T,Hirano K,Nagata T,Tsukada K Anticancer Res. 2015 Feb;35(2):719-27 |
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LOT Information
| Cell No. | JCRB1584 | Cell Name | TYUC-1-Luc |
|---|---|---|---|
| LOT No. | 05232014 | Lot Specification | distribution |
| Medium | DMEM and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 3.08-3.89x10^5 cells/ml | Methods for Passages | Dilution(Centrifugage at 100-300xg for 3-5 minites to remove dead cells) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.52x10^6 |
| Viability at cell freezing (%) | 86.5 | Antibiotics Used | free |
| Passage Number | p6* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:8 D7S820:10 D16S539:11 VWA:16 TH01:9 AM:X TPOX:8 CSF1PO:11 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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