JCRB1628 iPS-TIG107-4f1
Cell information
Important Notice(s)1vialあたり6well plateで 1～2wellに解凍してください。
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1628 | Cell Name | iPS-TIG107-4f1 |
|---|---|---|---|
| Profile | Human iPS cell line which was generated from adult human skin fibroblast cell line (JCRB0532: TIG-107). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 81-year-old |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Transgene: Human OCT3/4, SOX2, KLF4, and c-MYC (in pMXs retroviral vectors) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | human ES-like | Character | |
| Classify | transformed | Established by | Yamanaka, S. |
| Registered by | Yamanaka, S. | Regulation for Distribution | 1) Academic research purpose only. 2) Conclude a MTA with Kyoto Univ.. |
| Comment | Year | 2009 | |
| Medium | Primate ES cells medium (ReproCELL) supplemented 4 ng/mL bFGF. | Methods for Passages | Cells are treated with the solution consiseted by 0.25% Trypsin, 0.1 mg/mL collagenase IV, 1mM CaCl2, and 20% KSR. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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| PDF File1 PDF File2 PDF File3 PDF File4 |
LOT Information
| Cell No. | JCRB1628 | Cell Name | iPS-TIG107-4f1 |
|---|---|---|---|
| LOT No. | 07012015 | Lot Specification | distribution |
| Medium | Culture medium for human iPS cells (attachmentA) ,FGF-2 4ng/ml | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Cells were harvested after treatment with CTK.(Split ratio=1/1-1/13) | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.67x10^5 |
| Viability at cell freezing (%) | 44.9 | Antibiotics Used | |
| Passage Number | p7+5+4 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:11,12 D7S820:8,10 D16S539:13 VWA:14,18 TH01:6,9 AM:X TPOX:8,9 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 10% DMSO,4ng/ml FGF-2,10uM ROCK inhibitor in Culture medium for human iPS cells(attachmentB) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
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| No image |
| PDF File1 PDF File2 |