JCRB1630 DR-EcoScreen
Cell information
Important Notice(s)JCRB1630 DR-EcoScreen細胞の分譲について
On the Distribution of DR-EcoScreen (JCRB1630)
Cell type：genetically-modified cells (View Pricing Information)
| JCRB No. | JCRB1630 | Cell Name | DR-EcoScreen |
|---|---|---|---|
| Profile | DR-EcoScreen is for evaluation of a rapid in vitro aryl hydrocarbon receptor tarnscriptional activation assay. | Other Name | |
| Animal | mouse | Strain | C57L |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | This cell line was stably transfected with a reportor plasmid, pIND-DCDR7 (dioxin-responsive element linked to luciferase). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Hygromycin-resistant |
| Classify | transformed | Established by | Iida, M. |
| Registered by | Kojima, H. | Regulation for Distribution | MTA between applicant and developer is required. |
| Comment | Year | 2014 | |
| Medium | Alpha-MEM with 5% FBS. 0.15mg/ml hygromycin is recommended to be added during cultivation and maintenance. (Hygromycin should be removed at the assay of test samples.) | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA. |
| Cell Number on Passage | split ratio 1/10 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | Liver (Hepa1c1c7 cell derivative) |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:18835618 | In vitro screening for aryl hydrocarbon receptor agonistic activity in 200 pesticides using a highly sensitive reporter cell line, DR-EcoScreen cells, and in vivo mouse liver cytochrome P450-1A induction by propanil, diuron and linuron. Takeuchi S,Iida M,Yabushita H,Matsuda T,Kojima H Chemosphere. 2008 Dec;74(1):155-65 |
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LOT Information
| Cell No. | JCRB1630 | Cell Name | DR-EcoScreen |
|---|---|---|---|
| LOT No. | 06292015 | Lot Specification | distribution |
| Medium | MEM alpha (GIBCO) with 5% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.12-7.52x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.67x10^6 |
| Viability at cell freezing (%) | 99.0 | Antibiotics Used | free |
| Passage Number | p8* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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