JCRB1668 TYEK-1
Cell information
Important Notice(s)On the MTA of this cell line with Kyoto University (for profit organizations in Japan)
Announcement of unavailability of this cell line to overseas researcher outside of Japan
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1668 | Cell Name | TYEK-1 |
|---|---|---|---|
| Profile | Human poorly differentiated squamous cell carcinoma cell line established from pharyngeal cancer. 5Fu, PTX sensitive. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 65-year-old |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | pharyngeal cancer, squamous cell carcinoma (poorly differentiated) | Metastasis | |
| Tissue Metastasized | Genetics | p53 mutation exon 6 codon 214 (GAT to CGT). chromosone: 62-66, modal number was 63. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | T4M0N1 stage 4b, 5Fu and PTX sensitive. avairable for xenograft. |
| Classify | tumor | Established by | Shimada, Y. |
| Registered by | Shimada, Y. | Regulation for Distribution | |
| Comment | Year | 2016 | |
| Medium | Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal calf serum. The use of collagen-coated culture vessel to culture is desirable.. | Methods for Passages | Cells harvested after treatment with trypsin and EDTA. |
| Cell Number on Passage | split ratio 1/10 (DT 19.8h) | Race | |
| CO2 Conc. | 5% | Tissue Sampling | pharynx |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1668 | Cell Name | TYEK-1 |
|---|---|---|---|
| LOT No. | 04072016 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA12J396) on collagen coated dish | Temperature | 37 C |
| Cell Density at Seeding | 0.83-5.3x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/10) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.6x10^6 |
| Viability at cell freezing (%) | 99.00 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:11 D7S820:9,10 D16S539:9,10 VWA:14,18 TH01:6,9 AM:X TPOX:8,11 CSF1PO:10 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | When cells are cultured on collagen coated dish split ratio is 1/10, if not split ratio is 1/4. |
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