JCRB1683 HARA CMV-Luc
Cell information
Important Notice(s)発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines
Information for introduced genes, etc in luciferase expressing cell lines
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1683 | Cell Name | HARA CMV-Luc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line (HARA;Human lung squamous cell carcinoma with PTHrP expression cell line). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 57 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | Metastasis | Y | |
| Tissue Metastasized | Genetics | pLVSIN-luc transfected HARA cells. Luciferase stably expressing cell line. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | This cell line expresses PTHrP highly and the bone metastasis is induced extensively by the injection to the heart chamber of nude mouse. Hypercalcemia is induced in mice by hypodermic transplantation of the cell. Puromycin-resistant. |
| Classify | transformed | Established by | Murakami, T. |
| Registered by | Murakami, T. | Regulation for Distribution | To exchange and follow MTA between oversea end-user and cell-developer. |
| Comment | Year | 2016 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA. Medium change every 3 days. |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | pleural fluid |
| Tissue Type | human squamous cell lung carcinoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
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LOT Information
| Cell No. | JCRB1683 | Cell Name | HARA CMV-Luc |
|---|---|---|---|
| LOT No. | 06172016 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.01x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.36x10^6 |
| Viability at cell freezing (%) | 98 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:9 D7S820:12 D16S539:10 VWA:16,17 TH01:7 AM:X,Y TPOX:8,9 CSF1PO:13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 89 | Additional information |
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