JCRB1691 RERF-LC-Sq1 CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1691 Cell Name   RERF-LC-Sq1 CMV-Luc
Profile   Luciferase stably expressing cell line (RERF-LC-Sq1;Cell line established from human lung carcinoma tissue). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   74
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer, squamous carcinoma Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Established from human lung squamous carcinoma tissue grown in SCID mice. Puromycin-resistant.
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. Methods for Passages   Cells harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 minutes. Subculture once in two weeks.
Cell Number on Passage   split ratio = 1:4 Race   Japanese
CO2 Conc.   5% Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG36
Images
Movies



LOT Information

Cell No.   JCRB1691 Cell Name   RERF-LC-Sq1 CMV-Luc
LOT No.   08242016 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.5-5.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.60x10^6
Viability at cell freezing (%)   96.44 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:11,12
D16S539:9,11
VWA:17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
CLOSE