JCRB1741 XP3R

Cell information

Cell type：human iPS cells (View Pricing Information)

JCRB No.   JCRB1741 Cell Name   XP3R
Profile   iPS cell line derived from human Xeroderma Pigmentosum-A patient skin fibroblast cells (XP3OS), infected with recombinant retroviruses expressing the four factors (Oct4, Sox2, Klf4 and c-Myc). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   passage 11 (deposit)
Classify   transformed Established by   Shunsuke Yuba
Registered by   Ryoji Chubachi Regulation for Distribution   
Comment    Year   2017
Medium   DMEM/F-12 containing knockout serum replacement (KSR), 0.1 mM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) Methods for Passages   iPSCs were passed using dissociation solution (0.25% trypsin, 0.1 mg/ml collagenase type IV, 10 mM CaCl2, and 20% KSR in distilled water). split ratio=1/3
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_9S65
Reference
Pubmed id:26090552Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model.
Ohnishi H,Kawasaki T,Deguchi T,Yuba S
Cell Reprogram. 2015 Aug;17(4):268-74

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LOT Information

Cell No.   JCRB1741 Cell Name   XP3R
LOT No.   07282017 Lot Specification   distribution
Medium   DMEM/F-12 containing 20% SSR(Wako),0.1mM NEAA,0.1mM 2-mercaptoethanol and 5ng/ml bFGF on mitomycin C-treated MEF feeder cells (ICR) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were harvested after treatment with CTK(0.25% trypsin, 0.1 mg/ml collagenase type IV, 10 mM CaCl2 and 20% KSR).Split ratio=1/3-1/10
Doubling Time   NT Cell Number in Vial (cells/1ml)   NT
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p11+5 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9,11
D7S820:11,12
D16S539:9,12
VWA:14,16
TH01:7
AM:X
TPOX:8,11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   STEM-CELLBANKER(ZENOAQ) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Mitomycin C-treated MEFs are used as feeders. 1vial is 0.4ml.Type of ampoules is plastic.
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