JCRB1755 XP39R
Cell information
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1755 | Cell Name | XP39R |
|---|---|---|---|
| Profile | iPS cell line derived from human Xeroderma Pigmentosum-A patient skin fibroblast cells (XP39OS), infected with recombinant retroviruses expressing the four factors (Oct4, Sox2, Klf4 and c-Myc). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | passage 9 (deposit) | |
| Classify | transformed | Established by | Ohnishi, H. |
| Registered by | Shunsuke Yuba | Regulation for Distribution | |
| Comment | Year | 2017 | |
| Medium | DMEM/F-12 containing knockout serum replacement (KSR), 0.1 mM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) | Methods for Passages | iPSCs were passed using dissociation solution (0.25% trypsin, 0.1 mg/ml collagenase type IV, 10 mM CaCl2, and 20% KSR in distilled water). split ratio=1/3 |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
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-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:26090552 | Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model. Ohnishi H,Kawasaki T,Deguchi T,Yuba S Cell Reprogram. 2015 Aug;17(4):268-74 |
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LOT Information
| Cell No. | JCRB1755 | Cell Name | XP39R |
|---|---|---|---|
| LOT No. | 11022017 | Lot Specification | distribution |
| Medium | DMEM and Ham's F12 medium(1/1) with 20% SSR(Wako),0.1mM NEAA,0.1mM 2-mercaptoethanol and 5ng/ml bFGF on mitomycin C-treated MEF(ICR). | Temperature | 37 C |
| Cell Density at Seeding | NT | Methods for Passages | Cells were harvested after treatment with CTK(0.25% trypsin,0.1mg/ml collagenase type IV,10mM CaCl2 and 20% KSR) Split ratio=1/3-1/10 |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | NT |
| Viability at cell freezing (%) | NT | Antibiotics Used | |
| Passage Number | p9+5 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:9,11 D7S820:11,12 D16S539:10,12 VWA:17,18 TH01:6,7 AM:X TPOX:8,11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | STEM-CELLBANKER(ZENOAQ) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | Mitomycin C-treated MEFs are used as feeders. 1vial is 0.4ml. Type of ampoules is plastic. |
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