JCRB1839 AF-T21-iPS#6
Cell information
Important Notice(s)About the chromosome analysis of AF-T21-iPS#6: loss of one X chromosome during cultivation.
AF-T21-iPS#6の染色体分析結果について： X染色体1本の脱落の問題]
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1839 | Cell Name | AF-T21-iPS#6 |
|---|---|---|---|
| Profile | Amniotic fluid-derived iPS cell line in human Down syndrome patient. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | Pregnancy 33 |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Down syndrome | Metastasis | N |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | human ES cell like | Character | 21 trisomic iPS cell line. The total number of chromosomes changed during serial passages. 5th passage: 47 (information by depositor) 28th passage: 47 (62%), 46 (38%), (tested by JCRB) 66th passage: 46 (information by depositor) |
| Classify | transformed | Established by | Umezawa, A. |
| Registered by | Umezawa,A. | Regulation for Distribution | |
| Comment | Pregnancy 33 weeks 4 days | Year | 2019 |
| Medium | Essential 8 (Invitrogen) | Methods for Passages | Usung 0.5mM EDTA in PBS and cultured on 0.5ug/sq.cm VTN-N;Vitronectin ,truncated recombinant human (Invitrogen) coated dishes. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | Amniotic fluid |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:30760866 | Autonomous trisomic rescue of Down syndrome cells. Inoue M,Kajiwara K,Yamaguchi A,Kiyono T,Samura O,Akutsu H,Sago H,Okamoto A,Umezawa A Lab Invest. 2019 Jun;99(6):885-897 |
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LOT Information
| Cell No. | JCRB1839 | Cell Name | AF-T21-iPS#6 |
|---|---|---|---|
| LOT No. | 12222020 | Lot Specification | distribution |
| Medium | Essential 8 (Invitrogen) | Temperature | 37 C |
| Cell Density at Seeding | NT | Methods for Passages | Usung 0.5mM EDTA in PBS and cultured on 0.5ug/sq.cm VTN-N;Vitronectin ,truncated recombinant human (Invitrogen) coated dishes. 1/20-1/50 split every 4-5days |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.1x10^6 /0.5ml/vial |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | p24(21+3) | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:11,12 D7S820:10,12 D16S539:9,11 VWA:14,19 TH01:6,7 AM:X TPOX:10,12 CSF1PO:10 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | STEM-CELLBANKER GMP Grade(ZENOAQ) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 3.1x10^6 /vial | Additional information | Start cell culture 0.5-2x10^5 cells in VTN-N coated 60mmdish using 10uM Y-27632.Medium renewal next day to remove Y-27632. |
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