JCRB3004 Dch4F
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3004 | Cell Name | Dch4F |
|---|---|---|---|
| Profile | Human primary cell from Dyschromatosis (dominant) patient. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Dyschromatosis | Metastasis | |
| Tissue Metastasized | Genetics | Dyschromatosis | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | skin fibroblast |
| Classify | primary | Established by | Sasaki,MS. |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | father of Dch4 | Year | 2006 |
| Medium | RPMI1640 medium with 20% fetal calf serum | Methods for Passages | Cells were treated with 0.25% trypsin and 0.02% EDTA to harvest cells. |
| Cell Number on Passage | 2 fold dilution | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB3004 | Cell Name | Dch4F |
|---|---|---|---|
| LOT No. | 06182007 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) with 20% bovine serum(Hyclone GRD0051,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 5.2x10^3 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.7x10^6 |
| Viability at cell freezing (%) | 96.4 | Antibiotics Used | free |
| Passage Number | p7 | PDL | 6.7 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:8,10 D7S820:8,11 D16S539:9,10 VWA:14,18 TH01:6 AM:X,Y TPOX:9,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker:BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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