JCRB3036 MK-51

Cell information

Cell type:human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB3036 Cell Name   MK-51
Profile   Congenital anomaly (Mutant karyotype) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   blood
Case history    Metastasis   
Tissue Metastasized    Genetics   48 XXXX
Life Span   finite Crisis PDL   
Morphology   lymphocyte-like Character   lymphocyte isolated by Ficoll-Hypaque
Classify    Established by   
Registered by   Radiation Biology Center, Kyoto University Regulation for Distribution   
Comment    Year   
Medium    Methods for Passages   
Cell Number on Passage    Race   Japanese
CO2 Conc.    Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG77
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LOT Information

Cell No.   JCRB3036 Cell Name   MK-51
LOT No.   03102016 Lot Specification   distribution
Medium   1st culture ,TLY CULTURE kit 25(Cosmobio) after that,RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and IL-2(100U/ml)(Roche Interleukin-2,human) Temperature   37 C
Cell Density at Seeding   1.5-3.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.05x10^6
Viability at cell freezing (%)   92.95 Antibiotics Used   
Passage Number   p4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:10,12
D7S820:10,12
D16S539:10
VWA:16,18
TH01:6,7
AM:X
TPOX:8
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5 %
Viability immediately after thawing (%)   NT Additional information   This cell is not infinite. Type of ampoules is plastic.
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