JCRB3041 Werner
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3041 | Cell Name | Werner |
|---|---|---|---|
| Profile | Hereditary disease Werner syndrome (autosomal recessive) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | ||
| Case history | Werner syndrome | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | Crisis PDL | ||
| Morphology | Character | ||
| Classify | primary | Established by | SASAKI M. |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | Year | ||
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | Tissue Sampling | ||
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB3041 | Cell Name | Werner |
|---|---|---|---|
| LOT No. | 10272016 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA12J396,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 1.1-1.5x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3-1/4) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.50x10^6 |
| Viability at cell freezing (%) | 91.7 | Antibiotics Used | free |
| Passage Number | p7 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:8,12 D7S820:8 D16S539:9,12 VWA:18 TH01:9 AM:X,Y TPOX:8 CSF1PO:12,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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| Cell No. | JCRB3041 | Cell Name | Werner |
|---|---|---|---|
| LOT No. | 02242023 | Lot Specification | distribution |
| Medium | RPMI1640 medium (GIBCO Cat#11875) with 20% fetal bovine serum（non HI) (Biowest cat.No. S1820-500 LotNo.S09336S1820) | Temperature | 37 C |
| Cell Density at Seeding | 2.5-4.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/2.5-1/3) Be careful not to lower density. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.90x10^6 |
| Viability at cell freezing (%) | 92.6 | Antibiotics Used | free |
| Passage Number | p11 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,13 D13S317:8,12 D7S820:8 D16S539:9,12 VWA:18 TH01:9 AM:X,Y TPOX:8 CSF1PO:12,13 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 91.5 | Additional information | Be careful not to lower density. |
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