JCRB3043 KH-AML
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3043 | Cell Name | KH-AML |
|---|---|---|---|
| Profile | Hereditary disease Fanconi anemia (autosomal recessive) Acute myelogenous leukemia cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | |
| Identity | Tissue for Primary Cancer | ||
| Case history | Acute myelogenous leukemia(AML) | Metastasis | |
| Tissue Metastasized | Genetics | GM-CSF dependance | |
| Life Span | Crisis PDL | ||
| Morphology | Character | leukemia cell line of KURB1546 AP37P | |
| Classify | primary | Established by | SASAKI M. |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | GM-CSF dependance | Year | |
| Medium | RPMI1640 medium with 15% heat inactivated fetal bovine serum and 10ng/ml GM-CSF | Methods for Passages | |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | Tissue Sampling | ||
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
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LOT Information
| Cell No. | JCRB3043 | Cell Name | KH-AML |
|---|---|---|---|
| LOT No. | 04172017 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 15% heat inactivated fetal bovine serum(SIGMA12J396) and 10ng/ml GM-CSF | Temperature | 37 C |
| Cell Density at Seeding | 1-3x10^5 cells/ml | Methods for Passages | dilution(1/3dilution) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.83x10^6 |
| Viability at cell freezing (%) | 98.3 | Antibiotics Used | free |
| Passage Number | p6* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12,13 D13S317:9,12 D7S820:10,13 D16S539:9 VWA:17 TH01:7,9 AM:X,Y TPOX:8,11 CSF1PO:11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | GM-CSF dependent |
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