JCRB3063 AP39P(L)
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3063 | Cell Name | AP39P(L) |
|---|---|---|---|
| Profile | Hereditary disease Aplastic anemia (autosomal recessive) High SCE (sister chromatid exchange) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | blood | |
| Case history | Aplastic anemia (High SCE) | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | Crisis PDL | ||
| Morphology | lymphoblast-like | Character | EBV-transformed lymphoblastoid cell |
| Classify | transformed | Established by | Sasaki M. |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | 2nd | Year | 2006 |
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | Tissue Sampling | blood | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
LOT Information
| Cell No. | JCRB3063 | Cell Name | AP39P(L) |
|---|---|---|---|
| LOT No. | 09132019 | Lot Specification | distribution |
| Medium | RPMI 1640 medium (GIBCO Cat. # 11875) with 15% heat inactivated fetal bovine serum (SIGMA12J396 ) | Temperature | 37 C |
| Cell Density at Seeding | 1.5 - 7.5 x 10^5 cells/mL | Methods for Passages | Dilution (split ratio 1:4) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.8 x 10^6 |
| Viability at cell freezing (%) | 96.3 | Antibiotics Used | free |
| Passage Number | p3* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:9,11 D13S317:10,12 D7S820:9,10 D16S539:10,12 VWA:16,17 TH01:6,9 AM:X TPOX:8,11 CSF1PO:11,12 |
|
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |