JCRB9004 HeLa

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9004 Cell Name   HeLa
Profile   The first aneuploid, epitherial-like cell line(Feb. 1951) from human tissue and maintained continuously by serial cell culture. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   31
Identity   available Tissue for Primary Cancer   uterine cervix
Case history   carcinoma of cervix Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   first establish cell line in vitro from human
Classify   tumor Established by   Gey,Coffma,Kubicek
Registered by   JCRB Regulation for Distribution   Not regulated
Comment   EV=1.000 against other HeLa cells, like HeLaP3, HeLa229, and etc. Prepared from CCL 2. Year   1985
Medium   Eagle's minimum essential medium (Earle's salts, containing L-glutamine) with non essential amino acids and 10 % fetal bovine serum. Methods for Passages   Cells harvested after treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/10 Race   Black
CO2 Conc.   5 % Tissue Sampling   cervix
Tissue Type   epitheloid carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes The HPV18 gene integrated to host cells has deletion, and viral particle is not produced by the cells.

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0030
Reference
Pubmed id:11792820Identification of essential genes in cultured mammalian cells using small interfering RNAs.
Harborth J,Elbashir SM,Bechert K,Tuschl T,Weber K
J Cell Sci. 2001 Dec;114(Pt 24):4557-65
Pubmed id:11438719Cell culture forensics.
O'Brien SJ
Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7656-8
Pubmed id:11373684Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
Elbashir SM,Harborth J,Lendeckel W,Yalcin A,Weber K,Tuschl T
Nature. 2001 May 24;411(6836):494-8
Pubmed id:9892199Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping.
Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T
Cancer Res. 1999 Jan 1;59(1):141-50
Pubmed id:9215212Record of the first physician to see Henrietta Lacks at the Johns Hopkins Hospital: history of the beginning of the HeLa cell line.
Jones HW Jr
Am J Obstet Gynecol. 1997 Jun;176(6):S227-8
Pubmed id:3691964History of the acceptance of human diploid cell strains as substrates for human virus vaccine manufacture.
Hayflick L,Plotkin S,Stevenson RE
Dev Biol Stand. 1987;68():9-17
Pubmed id:13745868Animal cell cultures.
SALZMAN NP
Science. 1961 May 19;133(3464):1559-65
Pubmed id:13052828Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix.
SCHERER WF,SYVERTON JT,GEY GO
J Exp Med. 1953 May;97(5):695-710


Research results by users. Click！
Pubmed id:28614654 Creation of a Synthetic Ligand for Mitochondrial DNA Sequence Recognition and Promoter-Specific Transcription Suppression.
Hidaka T,Pandian GN,Taniguchi J,Nobeyama T,Hashiya K,Bando T,Sugiyama H
J Am Chem Soc. 2017 Jun 28;139(25):8444-8447

Images
Movies



LOT Information

Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   10252012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids (0.1 mM each) and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.4 - 6.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 19 - 23 hrs Cell Number in Vial (cells/1ml)   5.3 x 10^5
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   P114 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM,NEAA CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   
LOT No.   08112010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3.1 - 7.1 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 24 hrs Cell Number in Vial (cells/1ml)   9.0 x 10^5
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P114 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   
LOT No.   07312008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids (0.1 mM each) and 10% fetal bovine serum (GIBCO Cat. # 10099. lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   98.8 Antibiotics Used   free
Passage Number   P115 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   
LOT No.   04222006 Lot Specification   distribution
Medium   Eagle's minimam essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Subcultue twice a week.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P114 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type A), MD. LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBC- culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   
LOT No.   03112004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS Lot; GIBCO3762782S) Temperature   37 C
Cell Density at Seeding   3-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 22 hrs Cell Number in Vial (cells/1ml)   7.7 x 10^5
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   P111 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - basal medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   05232001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with NEAA and 10% fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.5 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 5 minutes. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.4 x 10^5
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P114 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, LDH and G6PD(typeA) examined. Human, HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   071994 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% heat-inactivated FBS/0070893 Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Washed cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5-8 min. at 37 C.
Doubling Time   23.6 hrs Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   P110 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12,14
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   032593 Lot Specification   distribution
Medium   Eagle's minimal essential medium (Nissui) with non-essential amino acids(GIBCO 100x) and 10% FBS Temperature   37 C
Cell Density at Seeding   1.23x10^5 cells/ml Methods for Passages   Wash cells once with PBS(-) and treat with 0.25%- trypsin and 0.02%-EDTA for 5 min. at 37 C.
Doubling Time   27hrs Cell Number in Vial (cells/1ml)   1.92x10^6
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   P110 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   032592 Lot Specification   distribution
Medium   EMEM + non essential A.A + FBS 10%(lot;J.R.Scientific,DO 01400 Temperature   37 C
Cell Density at Seeding   2.2x10^5 Methods for Passages   0.25% trypsin + 0.02% EDTA
Doubling Time   12hr Cell Number in Vial (cells/1ml)   3.9x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P110 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   100490 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P110 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   020689 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P110 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   092286 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P110 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   
LOT No.   071596 Lot Specification   distribution
Medium   Eagle's MEM with non-essential amino acids and 10%fetal calf serum (FCS lot; BioWhittaker 3M0581) Temperature   37 C
Cell Density at Seeding   8-9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8.8 x 10^5
Viability at cell freezing (%)   82.3 Antibiotics Used   free
Passage Number   P111 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type A), MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   
LOT No.   07272000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   4-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.= 30-35 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P119 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   08072015 Lot Specification   distribution
Medium   Eagle's minimum essential medium (Earle's salts, containing L-glutamine) with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1.4 - 6.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   25 - 28 hrs Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   P114 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   96.6 Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   06012016 Lot Specification   distribution
Medium   Eagle's minimum essential medium (Earle's salts, containing L-glutamine) with non-essential amino acids and 10 % fetal bovine serum. Temperature   37 C
Cell Density at Seeding   1.9 - 2.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P115 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   07262017 Lot Specification   distribution
Medium   Eagle's minimum essential medium(containing L-glutamine) with non essential amino acid and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.3-2.6x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/2-1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   99.00 Antibiotics Used   free
Passage Number   p114 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Images
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   08162018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) and non-essential amino acids. Temperature   37 C
Cell Density at Seeding   1.2 - 3.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P115 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   98.3 Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   07072020 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) with non essential amino acid (GIBCO cat.No.11140-050) and 10% heat inactivated fetal bovine serum(SIGMA cat.No.172012 Lot No.12J396) Temperature   37 C
Cell Density at Seeding   0.348-1.35x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.35x10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   p114 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   92.0 Additional information   
Images
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   12212020 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.4 - 9.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P115 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   98.9 Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   04152022 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   4.3 - 5.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P114 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   07282023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   P115 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   97.6 Additional information   
Cell No.   JCRB9004 Cell Name   HeLa
LOT No.   11062023 Lot Specification   distribution
Medium   MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids (nakarai tesque #21443-15) and and 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   6.9-7.6x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with with 0.25% trypsin and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.94x10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   p115 PDL   NT
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   95.95 Additional information   
Images
CLOSE