JCRB9005 BALB/3T3 clone A31 Cell information
Cell type:general cells
(View Pricing Information )
JCRB No.
JCRB9005
Cell Name
BALB/3T3 clone A31
Profile
fibroblast, contact inhibited, (BALB/c mouse)
Other Name
BALB/3T3
Animal
mouse
Strain
BALB/c
Genus
Mus
Species
musclus
Sex
Age
Identity
Tissue for Primary Cancer
embryo
Case history
Metastasis
Tissue Metastasized
Genetics
Life Span
Crisis PDL
Morphology
Character
contact inhibited
Classify
Established by
Aaronson & Todaro
Registered by
Mizusawa,H.
Regulation for Distribution
Comment
Prepared from CCL163.
Year
1985
Medium
Dulbecco's modified Eagle's medium with 10% calf serum
Methods for Passages
0.02% EDTA & 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Cell Number on Passage
3x10^5 cells/60mm dish
Race
CO2 Conc.
5 %
Tissue Sampling
embryo
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:7394516 Cell variants showing differential susceptibility to ultraviolet light--induced transformation. Kakunaga T,Crow JD Science. 1980 Jul 25;209(4455):505-7
Pubmed id:4311790 Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. Jainchill JL,Aaronson SA,Todaro GJ J Virol. 1969 Nov;4(5):549-53
Pubmed id:4301006 Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. Aaronson SA,Todaro GJ J Cell Physiol. 1968 Oct;72(2):141-8
Pubmed id:13985244 Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. TODARO GJ,GREEN H J Cell Biol. 1963 May;17():299-313
LOT Information
Cell No.
JCRB9005
Cell Name
LOT No.
11202009
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)
Temperature
37 C
Cell Density at Seeding
approx. 2 - 3 x 10^4 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA
Doubling Time
approx. 1 day
Cell Number in Vial (cells/1ml)
2.1 x 10^6
Viability at cell freezing (%)
93.7
Antibiotics Used
free
Passage Number
P72
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - DMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
LOT No.
9005
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% calf serum
Temperature
37C
Cell Density at Seeding
1 x 10^4 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA in PBS
Doubling Time
16hr
Cell Number in Vial (cells/1ml)
1.2x 10^5
Viability at cell freezing (%)
90
Antibiotics Used
free
Passage Number
P72
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD MD
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
70% Culture medium + 20% FCS + 10% DMSO
CO2 Conc.
5%
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
LOT No.
12102007
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% calf serum
Temperature
37C
Cell Density at Seeding
1x10^4 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA in PBS
Doubling Time
16hr
Cell Number in Vial (cells/1ml)
6.0 x 10^5
Viability at cell freezing (%)
91
Antibiotics Used
free
Passage Number
P72
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD MD
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
70% Culture medium + 20% CS + 10% DMSO
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
LOT No.
08072003
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% calf serum
Temperature
37 C
Cell Density at Seeding
2 x 10^4 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA
Doubling Time
D.T. = ca. 24 hrs
Cell Number in Vial (cells/1ml)
7.3 x 10^5
Viability at cell freezing (%)
92.7
Antibiotics Used
free
Passage Number
P72
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% CS - DMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
111695
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's minimal medium with 10% calf serum (ICN Flow 1059023).
Temperature
37 C
Cell Density at Seeding
8.4x10^4 cells/ml
Methods for Passages
Wash cells with PBS(-) and treated with 0.25%-trypsin and 0.02% EDTA for 10min. at 37 C.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
1.9x10^6
Viability at cell freezing (%)
97.0
Antibiotics Used
free
Passage Number
P70
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
Culture medium with 5% DMSO.
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
F00963
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% fetal calf serum (BioWhittaker, 3MC0581)
Temperature
37 C
Cell Density at Seeding
2.5-3.2x10^4 cells/ml
Methods for Passages
Cells were treated with 0.02% EDTA and 0.25% trypsin (Difco 1:250)
Doubling Time
NT
Cell Number in Vial (cells/1ml)
7.2x10^5
Viability at cell freezing (%)
77.2
Antibiotics Used
free
Passage Number
P69
PDL
NT
Sterility: MYCOPLASMA
NT
Sterility: BACTERIA
NT
Sterility: FUNGI
NT
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
090993
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% calf serum (Hyclone, 2111480)
Temperature
37 C
Cell Density at Seeding
7-8x10^4 cells/ml
Methods for Passages
Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5-15min. at 37 C.
Doubling Time
15.5 hours.
Cell Number in Vial (cells/1ml)
1.5x10^6
Viability at cell freezing (%)
96.5
Antibiotics Used
free
Passage Number
P70
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
NT
Isozyme Analysis
G6PD, LD, Pep B
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
082493
Lot Specification
distribution
Medium
Cells discarded.
Temperature
Cell Density at Seeding
Methods for Passages
Doubling Time
Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)
Antibiotics Used
Passage Number
PDL
Sterility: MYCOPLASMA
NT
Sterility: BACTERIA
Sterility: FUNGI
Isozyme Analysis
G6PD, LD, PepB
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
101691
Lot Specification
distribution
Medium
DMEM + CS 10%
Temperature
37 C
Cell Density at Seeding
3x10^5/6D
Methods for Passages
0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
1.0x10^6
Viability at cell freezing (%)
NT
Antibiotics Used
free
Passage Number
P71
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
Chromosome Information
NT
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
100890
Lot Specification
distribution
Medium
DMEM + CS 10%
Temperature
37
Cell Density at Seeding
3x10^5/6D
Methods for Passages
0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
1.5x10^6
Viability at cell freezing (%)
NT
Antibiotics Used
free
Passage Number
P71
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
NT
Sterility: FUNGI
NT
Isozyme Analysis
NT
Chromosome Mode
Chromosome Information
NT
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
090888
Lot Specification
distribution
Medium
DMEM + CS 10%
Temperature
37
Cell Density at Seeding
3x10^5/6D
Methods for Passages
0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
1.1x10^6
Viability at cell freezing (%)
NT
Antibiotics Used
free
Passage Number
P70
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
Chromosome Information
NT
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
03212013
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). The vial is incorrectly labeled as 03262013.
Temperature
37 C
Cell Density at Seeding
1 - 2.4 x 10^4 cells / mL
Methods for Passages
0.02% EDTA & 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time
Cell Number in Vial (cells/1ml)
7.1 x 10^5
Viability at cell freezing (%)
98.6
Antibiotics Used
free
Passage Number
p72
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
10% DMSO, 20% FBS, DMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
98.6
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
10062016
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Sigma Cat. # 172012)
Temperature
37 C
Cell Density at Seeding
approx. 3.5 x 10^4 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow cultures to become completely confluent.
Doubling Time
approx. 28 hrs
Cell Number in Vial (cells/1ml)
1.2 x 10^6
Viability at cell freezing (%)
97
Antibiotics Used
free
Passage Number
P72
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - DMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
97
Additional information
Cell No.
JCRB9005
Cell Name
BALB/3T3 clone A31
LOT No.
08062021
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500)
Temperature
37 C
Cell Density at Seeding
4.2 - 8.2 x 10^4 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 31 hrs.
Cell Number in Vial (cells/1ml)
1.2 x 10^6
Viability at cell freezing (%)
99.2
Antibiotics Used
free
Passage Number
P72
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
5% DMSO, 20% FBS - DMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
96.0
Additional information