JCRB9023 CCRF-CEM

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9023 Cell Name   CCRF-CEM
Profile   Human cell line established from patient with acute lymphoblastic leukemia. Other Name   CCRF-CEM
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   4
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   peripheral blood, acute lymphoblastic leukemia
Life Span   infinite Crisis PDL   
Morphology    Character   peripheral blood, acute lymphoblastic leukemia
Classify   tumor Established by   Foley,G.E. et al.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   A maximum of 1-2 x 10^6 cells/ml is obtainable. Year   1985
Medium   RPMI1640 medium with 20% fetal calf serum Methods for Passages   Collected by centrifugation,resuspended in every 2-3 days.
Cell Number on Passage   3-5 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood buffy coat
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0207
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905952
Reference
Pubmed id:3493047T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.
Drexler HG,Gaedicke G,Minowada J
Blut. 1987 Feb;54(2):79-87
Pubmed id:6312323Molecular characterization of genome of a novel human T-cell leukaemia virus.
Chen IS,McLaughlin J,Gasson JC,Clark SC,Golde DW
Nature. 1983 Oct 6-12;305(5934):502-5
Pubmed id:841332Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:4170381Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma.
Adams RA
Cancer Res. 1967 Dec;27(12):2479-82
Pubmed id:4295047Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster.
Adams RA,Foley GE,Uzman BG,Farber S,Lazarus S,Kleinman L
Cancer Res. 1967 Apr;27(4):772-83
Pubmed id:5224274Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope.
Uzman BG,Foley GE,Farber S,Lazarus H
Cancer. 1966 Nov;19(11):1725-42
Pubmed id:5320250Cytogenetic analysis of human lymphoblasts in continuous culture.
McCarthy RE,Junius V,Farber S,Lazarus H,Foley GE
Exp Cell Res. 1965 Oct;40(1):197-200

Images



LOT Information

Cell No.   JCRB9023 Cell Name   
LOT No.   04222005 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   7.9 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P6 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:9,13
D16S539:10,13
VWA:17,19
TH01:6,7
AM:X
TPOX:8
CSF1PO:10,11,12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9023 Cell Name   CCRF-CEM
LOT No.   061088 Lot Specification   distribution
Medium   RPMI 1640 80%, FCS 20% Temperature   37
Cell Density at Seeding   3-5 x 10^5/ml Methods for Passages   Collected by centrifugation,resuspended in every 2-3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.56x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *p3 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9023 Cell Name   CCRF-CEM
LOT No.   111185 Lot Specification   distribution
Medium   RPMI 1640 80%, FCS 20% Temperature   37
Cell Density at Seeding   3-5 x 10^5/ml Methods for Passages   Collected by centrifugation,resuspended in every 2-3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^7/ml
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P2* PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9023 Cell Name   CCRF-CEM
LOT No.   11292022 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   1.0 - 3.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   83.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:11,12
D7S820:9,13
D16S539:10,13
VWA:17,19
TH01:6,7
AM:X
TPOX:8
CSF1PO:10,11
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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