JCRB9036 104C1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9036 | Cell Name | 104C1 |
|---|---|---|---|
| Profile | fetal carcass, benzo(a)pyrene transformed, diploid | Other Name | |
| Animal | guinea pig | Strain | |
| Genus | Cavia | Species | porcellus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | fetal carcass, benzo(a)pyrene transformed, diploid | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | Established by | Evans C.H. | |
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from CRL1405. | Year | 1986 |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25 % trypsin. |
| Cell Number on Passage | split ratio=1/10 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | fetal body |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1260765 | Chromosome patterns (G and C bands) of in vitro chemical carcinogen-transformed guinea pig cells. Popescu NC,Evans CH,DiPaolo JA Cancer Res. 1976 Apr;36(4):1404-13 |
| Pubmed id:1116143 | Colony inhibition mediated by nonimmune leukocytes in vitro and skin reactivity in vivo as indices of tumorigenicity of guinea pig cultures transformed by chemical carcinogens. Evans CH,Cooney AM,DiPaolo JA Cancer Res. 1975 Apr;35(4):1045-52 |
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LOT Information
| Cell No. | JCRB9036 | Cell Name | 104C1 |
|---|---|---|---|
| LOT No. | 071195 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal bovine serum (ICN flow,1010183) | Temperature | 37 C |
| Cell Density at Seeding | 1.8x10^4cells/sq.cm. | Methods for Passages | Rinse once with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5 min. at 37 C. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.0x10^6 |
| Viability at cell freezing (%) | 99.0 | Antibiotics Used | free |
| Passage Number | P31 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LDH,NP |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |