JCRB9047 GH3 Cell information
Cell type:general cells
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JCRB No.
JCRB9047
Cell Name
GH3
Profile
pituitary tumor, somatotrophin and prolactin-secreting, (Wistar/Furth rat)
Other Name
Animal
rat
Strain
Wistar/Furth
Genus
Rattus
Species
norvegicus
Sex
F
Age
Identity
Tissue for Primary Cancer
pituitary
Case history
Metastasis
Tissue Metastasized
Genetics
Pituitary tumor, MtT/W5, Somatotropin secreting.
Life Span
Crisis PDL
Morphology
Character
Pituitary tumor, MtT/W5, Somatotrophin secreting.
Classify
Established by
Tashjian,A.H. et al.
Registered by
Regulation for Distribution
Following rules from ATCC
Comment
Prepared from CCL 82.1.
Year
1986
Medium
Ham's F10 medium 82.5% with 15% horse serum and 2.5% fetal calf serum.
Methods for Passages
Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage
split ratio=1/2 - 1/4
Race
CO2 Conc.
5 %
Tissue Sampling
pituitary gland
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:4951281 Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Tashjian AH Jr,Yasumura Y,Levine L,Sato GH,Parker ML Endocrinology. 1968 Feb;82(2):342-52
LOT Information
Cell No.
JCRB9047
Cell Name
LOT No.
10152012
Lot Specification
distribution
Medium
Ham's F10 medium with 15% heat-inactivated horse serum (GIBCO 26050) and 2.5% fetal bovine serum (GIBCO 10099.
Temperature
37 C
Cell Density at Seeding
3.2 - 8.5 x 10^5 clls/mL
Methods for Passages
Pipetting, or 0.25% trypsin and 0.02% EDTA. Cell attachment is weak. Most of cells are non-adherent.
Doubling Time
74 - 156 hrs
Cell Number in Vial (cells/1ml)
1.3 x 10^6
Viability at cell freezing (%)
82.4
Antibiotics Used
free
Passage Number
P30
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as rat by NP, G6PD, MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO - culture medium
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9047
Cell Name
GH3
LOT No.
101692
Lot Specification
distribution
Medium
Ham's F10 medium(Flow/Gibco) with 15% horse serum (JR Scientific, OD1223) and 2.5% FBS (Flow 047893)
Temperature
37 C
Cell Density at Seeding
1.5x10^5 cells/ml
Methods for Passages
Wash once with PBS(-) and treat with 0.25% trypsin and 0.02% EDTA for 10 min. at 37 C.
Doubling Time
26.8 hrs
Cell Number in Vial (cells/1ml)
2.27x10^6
Viability at cell freezing (%)
86.8
Antibiotics Used
free
Passage Number
P29
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
CO2 Conc.
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9047
Cell Name
GH3
LOT No.
050289
Lot Specification
distribution
Medium
Ham's F10, 82.5%; horse serum, 15%; FCS, 2.5%.
Temperature
37
Cell Density at Seeding
1/2 - 1/4
Methods for Passages
0.02% EDTA and 0.25% trypsin.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
2.8x10^6
Viability at cell freezing (%)
NT
Antibiotics Used
free
Passage Number
P29
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
Chromosome Information
NT
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9047
Cell Name
LOT No.
061797
Lot Specification
distribution
Medium
Ham's F10 medium with 15% horse serum and 2.5% fetal calf serum
Temperature
37 C
Cell Density at Seeding
2-3 x 10^5 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA. Cell attachment is very weak and most cells grow as suspension.
Doubling Time
D.T.=ca 3 days
Cell Number in Vial (cells/1ml)
2.1 x 10^6
Viability at cell freezing (%)
86.0
Antibiotics Used
free
Passage Number
P30
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
confirmed as rat by NP, G6PD, AST.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
CO2 Conc.
RFLP
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB9047
Cell Name
GH3
LOT No.
10272022
Lot Specification
distribution
Medium
Ham's F-10 medium with 15% horse serum (GIBCO Cat. #16050) and 2.5% fetal bovine serum (FBS; GIBCO Cat. # 10437)
Temperature
37 C
Cell Density at Seeding
6.3 - 7.5 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Some cells may form cell aggregate and not adhere to the substratum.
Doubling Time
approx. 52 hrs.
Cell Number in Vial (cells/1ml)
4.3 x 10^6
Viability at cell freezing (%)
96
Antibiotics Used
free
Passage Number
P29
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Y/N
Exoteric Gene
Medium for Freezing
10% DMSO in culture medium
CO2 Conc.
5%
RFLP
Viability immediately after thawing (%)
96
Additional information