JCRB9054 IMR-90

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9054 Cell Name   IMR-90
Profile   Normal human fibroblast. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   16week embryo
Identity   available Tissue for Primary Cancer   lung
Case history   normal Metastasis   
Tissue Metastasized    Genetics   fetal lung, diploid, female.
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   fetal lung, diploid, female.
Classify   normal Established by   Nichols, W.W. et al
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL186 (Population doubling is 24 at passage No.8.). Year   1986
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/5 Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0347
Reference
Pubmed id:841339Characterization of a new human diploid cell strain, IMR-90.
Nichols WW,Murphy DG,Cristofalo VJ,Toji LH,Greene AE,Dwight SA
Science. 1977 Apr 1;196(4285):60-3

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LOT Information

Cell No.   JCRB9054 Cell Name   
LOT No.   08172009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   8.6 x 10^5
Viability at cell freezing (%)   86.1 Antibiotics Used   free
Passage Number   P13 PDL   37
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9054 Cell Name   
LOT No.   02262008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   40 hrs Cell Number in Vial (cells/1ml)   1.6 x 106
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P13 PDL   38
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   050196 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as a human by LDH, NP and G6PD(human B).
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   010794 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10%fetal bovine serum/Hy11151031 Temperature   37 C
Cell Density at Seeding   9.8x10^5 cells/10cm dish Methods for Passages   Cells treated with 0.25% trypsin & 0.02% EDTA,theninoculated into gelatin or collagen coated dish.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P15 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   120887 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/5 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9054 Cell Name   
LOT No.   08042005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 43 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P17 PDL   37-38
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - MEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9054 Cell Name   
LOT No.   02292000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essenti-al amino acids and 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   split ratio=1:3-1:5 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 34 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P15 PDL   37-38
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   11082013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). Temperature   37C
Cell Density at Seeding   4.6 - 7.9 x 10^4 cells/mL Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   2.2 x 10^6 cells/1mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P15 PDL   PDL=43
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)   80.6 Additional information   
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   07212017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.5 - 1.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   P13 PDL   29.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   97.4 Additional information   
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   07052018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   87.6 Antibiotics Used   free
Passage Number   P13 PDL   30.9
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   87.6 Additional information   
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   09212021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids and 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   7.5 - 8.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P14 PDL   36
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   84 Additional information   
Cell No.   JCRB9054 Cell Name   IMR-90
LOT No.   02032023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   2.6 - 4.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   9.5 x 10^5
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P14 PDL   35.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM-NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
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