JCRB9055 R2C

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9055 Cell Name   R2C
Profile   Leydig cell testicular tumor,steroid secreting Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   testis
Case history    Metastasis   
Tissue Metastasized    Genetics   Leydig cell testicular tumor,steroid secreting
Life Span    Crisis PDL   
Morphology    Character   Leydig cell testicular tumor,steroid secreting
Classify    Established by   Shin, S. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 97. Year   1986
Medium   Ham's F10 medium 82.5% with 15% horse serum and 2.5% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/3 Race   
CO2 Conc.   5 % Tissue Sampling   testicular tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3571
Reference
Pubmed id:4868022Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells.
Shin SI,Yasumura Y,Sato GH
Endocrinology. 1968 Mar;82(3):614-6




LOT Information

Cell No.   JCRB9055 Cell Name   
LOT No.   04272005 Lot Specification   distribution
Medium   Ham's F10 with 15% horse serum and 2.5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Alternatively, cells can be harvested by pipetting.
Doubling Time   85 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO in culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9055 Cell Name   R2C
LOT No.   092992 Lot Specification   distribution
Medium   Ham's F10 medium (Flow,GIBCO) with 15% horse serum(JRS) and 2.5% fetal bovine serum(Flow 047893) Temperature   37 C
Cell Density at Seeding   1x10^5 cell/ml Methods for Passages   Wash cells with PBS(+), and treated with 0.25% collagenase type IV for 60 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   88.5 Antibiotics Used   free
Passage Number   P38 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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