JCRB9059 3T6-Swiss albino

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9059 Cell Name   3T6-Swiss albino
Profile   fibroblast, collagen-secreting, (Swiss albino mouse) Other Name   3T6
Animal   mouse Strain   Swiss albino
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   embryo, collagen-secreting
Life Span    Crisis PDL   
Morphology    Character   embryo, collagen-secreting
Classify    Established by   Todaro,J. & Green,H.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 96. Year   1987
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 - 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0601
Reference
Pubmed id:5971697Differentiated cell types and the regulation of collagen synthesis.
Green H,Goldberg B,Todaro GJ
Nature. 1966 Nov 5;212(5062):631-3




LOT Information

Cell No.   JCRB9059 Cell Name   
LOT No.   11142002 Lot Specification   distribution
Medium   Duobecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml (解凍時は75cm2への播種を推奨) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5.4 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P89 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9059 Cell Name   3T6-Swiss albino
LOT No.   020891 Lot Specification   distribution
Medium   DMEM + FCS 10% Temperature   37 C
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P88 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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