JCRB9063 WIL2-NS

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9063 Cell Name   WIL2-NS
Profile   B lymphocyte line, from patient with hereditary spherocytic anemia, non-immunoglobulin secreting variant Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   B lymphocyte, non-secreting variant
Life Span    Crisis PDL   
Morphology    Character   B lymphocyte, non-secreting variant
Classify    Established by   Levy,J.A. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL8155. A maximum of 1-2 x 10^6 cells/ml is obtainable. Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Dilution
Cell Number on Passage   1-2 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   lymphocyte
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2761
Reference
Pubmed id:804494Antibodies to cellular antigens in Sjögren's syndrome.
Alspaugh MA,Tan EM
J Clin Invest. 1975 May;55(5):1067-73
Pubmed id:4323138Further characterization of the WI-L1 and WI-L2 lymphoblastoid lines.
Levy JA,Buell DN,Creech C,Hirshaut Y,Silverberg H
J Natl Cancer Inst. 1971 Mar;46(3):647-54
Pubmed id:5673231Human lymphoblastoid lines from lymph node and spleen.
Levy JA,Virolainen M,Defendi V
Cancer. 1968 Sep;22(3):517-24

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LOT Information

Cell No.   JCRB9063 Cell Name   
LOT No.   08102000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2-10 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   6.5 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9063 Cell Name   WIL2-NS
LOT No.   103095 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat inactivated fetal bovine serum (ICN Flow 101083) Temperature   37 C
Cell Density at Seeding   1.2x10^5/m Methods for Passages   Cells are diluted to 1-3x10^5 cells/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.74x10^6
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11
D7S820:9,12
D16S539:11,12
VWA:17,20
TH01:8,10
AM:XY
TPOX:8,11
CSF1PO:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9063 Cell Name   WIL2-NS
LOT No.   061387 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   1-2 x 10^5/ml Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   99% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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