JCRB9082 COS-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9082 Cell Name   COS-1
Profile   SV40 transformed African green monkey kidney Other Name   
Animal   monkey, African green Strain   
Genus   Cercopithecus Species   aethiops
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   SV40 transformed African green monkey kidney
Life Span   infinite Crisis PDL   
Morphology    Character   SV40 transformed African green monkey kidney
Classify    Established by   
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1650. Year   1986
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/4 - 1/8 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0223
Reference
Pubmed id:2848247Isolation of a full-length cDNA insert encoding human aromatase system cytochrome P-450 and its expression in nonsteroidogenic cells.
Corbin CJ,Graham-Lorence S,McPhaul M,Mason JI,Mendelson CR,Simpson ER
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8948-52
Pubmed id:3869955Recombination and deletion of sequences in shuttle vector plasmids in mammalian cells.
Chakrabarti S,Joffe S,Seidman MM
Mol Cell Biol. 1985 Sep;5(9):2265-71
Pubmed id:6260373SV40-transformed simian cells support the replication of early SV40 mutants.
Gluzman Y
Cell. 1981 Jan;23(1):175-82

Images



LOT Information

Cell No.   JCRB9082 Cell Name   COS-1
LOT No.   08132010 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1-1.15x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.52x10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   p16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9082 Cell Name   
LOT No.   07042004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   0.5 - 1.0 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   27 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P18 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as African green monkey by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10%DMSO, 20% FBS - DMEM) CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9082 Cell Name   COS-1
LOT No.   092094 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium(low glucose) with 10% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   1-2x10^6/100mm dish Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA and inoculated.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.6x10^6
Viability at cell freezing (%)   91.4 Antibiotics Used   free
Passage Number   P18 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9082 Cell Name   COS-1
LOT No.   091492 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (070892,090292)+ 10% fetal bovine serum (Hyclone 11151031) Temperature   37 C
Cell Density at Seeding   1-1.75x10^5 cells/ml Methods for Passages   Cells are washed once with PBS, then treated with 0.25 % trypsin with 0.02% EDTA at 37 C for 15 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.14x10^6
Viability at cell freezing (%)   89.8 Antibiotics Used   free
Passage Number   P14 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9082 Cell Name   COS-1
LOT No.   100890 Lot Specification   distribution
Medium   DMEM + FCS 10% Temperature   37
Cell Density at Seeding   1/4 - 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9082 Cell Name   COS-1
LOT No.   091489 Lot Specification   distribution
Medium   DMEM + FCS 10% Temperature   37
Cell Density at Seeding   1/4 - 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9082 Cell Name   COS-1
LOT No.   030488 Lot Specification   distribution
Medium   DMEM + FCS 10% Temperature   37
Cell Density at Seeding   1/4 - 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9082 Cell Name   COS-1
LOT No.   110891 Lot Specification   distribution
Medium   D.MEM + FCS 10% Temperature   37 C
Cell Density at Seeding   1/4-1/8 Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P14 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9082 Cell Name   
LOT No.   061197 Lot Specification   distribution
Medium   Dulbecco's MEM with 10% fetal calf serum. Ampule label JCRB0253 is misprint. Temperature   37 C
Cell Density at Seeding   2-5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6.2 x 10^5
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as Afrikan green monkey by NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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