JCRB9103 KD

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9103 Cell Name   KD
Profile   fibroblast, lip Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity    Tissue for Primary Cancer   oral
Case history    Metastasis   
Tissue Metastasized    Genetics   lip, fibroblast
Life Span    Crisis PDL   
Morphology    Character   lip, fibroblast
Classify    Established by   Day,R.
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1295. Population doublings were about 38-40 at passage 3. Year   1986
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/2 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   lip
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2970
Reference
Pubmed id:7199389Variations in expression of mutant beta actin accompanying incremental increases in human fibroblast tumorigenicity.
Leavitt J,Bushar G,Kakunaga T,Hamada H,Hirakawa T,Goldman D,Merril C
Cell. 1982 Feb;28(2):259-68
Pubmed id:6892569Expression of a variant form of actin and additional polypeptide changes following chemical-induced in vitro neoplastic transformation of human fibroblasts.
Leavitt J,Kakunaga T
J Biol Chem. 1980 Feb 25;255(4):1650-61
Pubmed id:418410Neoplastic transformation of human diploid fibroblast cells by chemical carcinogens.
Kakunaga T
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1334-8
Pubmed id:4842250Studies on repair of adenovirus 2 by human fibroblasts using normal, xeroderma pigmentosum, and xeroderma pigmentosum heterozygous strains.
Day RS 3rd
Cancer Res. 1974 Aug;34(8):1965-70

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LOT Information

Cell No.   JCRB9103 Cell Name   
LOT No.   11052009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 27 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^5
Viability at cell freezing (%)   92.1 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   55
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9103 Cell Name   
LOT No.   06232003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = 51-70 hrs Cell Number in Vial (cells/1ml)   3.3 x 10^5
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   52
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9103 Cell Name   KD
LOT No.   032591 Lot Specification   distribution
Medium   Dulbecco`s modified eagles medium (Nissui Powd.) with 10% fetal bovine serum (JRS,CD19407) Temperature   37 C
Cell Density at Seeding   7.0x10^5 cells/ml Methods for Passages   Cells are harvested with 0.25% trypsin and 0.02% EDTA treatment.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1x10^5
Viability at cell freezing (%)   90.3 Antibiotics Used   free
Passage Number   *P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:10,12
D16S539:11,12
VWA:17
TH01:7,9
AM:X
TPOX:8,9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9103 Cell Name   KD
LOT No.   12112017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.3 - 3.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^5
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   54
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98.2 Additional information   
Cell No.   JCRB9103 Cell Name   KD
LOT No.   08262022 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO cat No. 11885-084) with 10% heat inactivated fetal bovine serum (Biowest cat.No. S1820-500 LotNo.S09336S1820). Temperature   37 C
Cell Density at Seeding   6.20-7.90x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/2-1/6)
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.21x10^5
Viability at cell freezing (%)   97.3 Antibiotics Used   free
Passage Number   p8* PDL   54
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:10,12
D16S539:11,12
VWA:17
TH01:7,9
AM:X
TPOX:8,9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   90.6 Additional information   
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