JCRB9108 J774A.1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9108 Cell Name   J774A.1
Profile   Mouse macrophage cell line. Other Name   
Animal   mouse Strain   BALB/c
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   monocyte-macrophage
Classify    Established by   Ralph,P.
Registered by    Regulation for Distribution   
Comment   Prepared from TIB 67. Year   1987
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Scraping. Remove old medium, add fresh, dislodge cells from substrate, and dispense into new flask.
Cell Number on Passage   split ratio = 1/3 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0358
Reference
Pubmed id:2110628Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells.
Bielecki J,Youngman P,Connelly P,Portnoy DA
Nature. 1990 May 10;345(6271):175-6
Pubmed id:3417786A prelysosomal compartment sequesters membrane-impermeant fluorescent dyes from the cytoplasmic matrix of J774 macrophages.
Steinberg TH,Swanson JA,Silverstein SC
J Cell Biol. 1988 Sep;107(3):887-96
Pubmed id:318922Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.
Ralph P,Nakoinz I
Cancer Res. 1977 Feb;37(2):546-50
Pubmed id:1101071Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line.
Ralph P,Nakoinz I
Nature. 1975 Oct 2;257(5525):393-4

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LOT Information

Cell No.   JCRB9108 Cell Name   
LOT No.   01182010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   Scraping by cell scraper.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   6.2 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9108 Cell Name   
LOT No.   09042009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Scraping by cell scraper.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   69 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9108 Cell Name   
LOT No.   02232007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   0.5-5 x 10^5 cells/ml Methods for Passages   scraping with rubber policeman (* after scraping, about half of cells cannot survive.)
Doubling Time   1-1.5 day Cell Number in Vial (cells/1ml)   4.0 x 10^6
Viability at cell freezing (%)   89.9 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP and G6PD, LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9108 Cell Name   J774A.1
LOT No.   042396 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   Confirmed as a mouse by LDH, NP and G6PD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9108 Cell Name   J774A.1
LOT No.   102392 Lot Specification   distribution
Medium   RPMI-1640 medium (090892) with 10% fetal bovine serum (JRH Biosciences 1B1062) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Cells were collected by scraping with rubber policeman and suspended in fresh medium.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   89.6 Antibiotics Used   free
Passage Number   *P7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9108 Cell Name   J774A.1
LOT No.   111888 Lot Specification   distribution
Medium   DMEM + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/6 Methods for Passages   Scraping. Remove old medium, add fresh, dislodge cells from the flask substrate, and dispense into new flasks.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9108 Cell Name   
LOT No.   020998 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-6 x 10^5 cells/ml Methods for Passages   Pipetting or scraping and dilution.
Doubling Time    Cell Number in Vial (cells/1ml)   5.1 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9108 Cell Name   J774A.1
LOT No.   02212019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.4 - 1.8 x 10^5 cells/mL Methods for Passages   Vigorus pipetting or scraping (scraping will produce many dead cells after passage)
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   2.9 x 10^6 (viable cells)
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   57.1 Additional information   Many dead cells (low viability) in ampoule is due to the scraping. The growth after thawing was observed to be no problem.
Cell No.   JCRB9108 Cell Name   J774A.1
LOT No.   01272021 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1280-500) Temperature   37 C
Cell Density at Seeding   1.6 - 3.3 x 10^5 cells/mL Methods for Passages   Cells were first treated with 0.125% trypsin, and then harvested by scraper.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
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