|Profile:Human thyroid carcinoma cell line.|
|Previous cell Number:||FDSC0042|
|Sex and Age:||F :44-year-old|
|Scientific Name:||Homo sapiens|
|Medium:||Dulbecco's modified Eagle's medium with 10% fetal calf serum.|
|Passage method:||Cells are treated with 0.02% EDTA and 0.25% trypsin|
|CO2 Concentration:||5 %|
|Cell no. at passage:||1-5x10^5 cells/10cm dish|
|Genetics:||thyroid carcinoma, female, 44-year-old|
|Characteristics:||thyroid carcinoma, female, 44-year-old|
|Cell ID data:||available|
|DNA Profile (STR):|| D5S818:11,12|
|Virus DNA detected:||CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] -- Description|
|Established by||Inoue,D. & Sugawa,H.|
|Special notice:||Report to Enomoto, T. is required before using.|
|Comments:||The HTC/C3 is unique by STR-PCR (04/22/2001).|
(* The format of the table was revised on Apr.25.2003.)
|Cells(1), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), STR-PCR(3), Freezing process, Others(1), Others(2),|
[ Lot No.: 04202001: seed (JCRB0164) ]|
Passage Number, P6*
Cell culture was started without cloning from the previous stock.
Date cell culture started:04/02/2001
Concentration of cells in an ampule: 1.3 x 10^6 cell/ml
Viability under microscope (%): 91.3
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD(typeB), NP, LDH examined. Human non HeLa..
Anchorage dependency: Yes
Cell Identification: available
RFLP data: Unique pattern was obtained.
[ Culture condition of this lot.]
Freezing medium: Culture medium containing 5% DMSO.
Passage method: Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA at 37 C for 10 minutes. Subculture once every 5-6 days and change medium once in between the subculture.
Growth temperature: 37 C
CO2 concentration: 5 %
Cell No. at passage: 7.0 x 10^4 cells/ml
Additional comments: This cell culture was devided into NIHS and JCRB becuase no Mycoplasmas contamination observed.
[ Additional Data ]
(Date downloaded: 2008-06-16)