JCRB0164:HTC/C3

JCRB0164 [HTC/C3]

Profile:Human thyroid carcinoma cell line.

Date accepted: 02/08/1990

Previous cell Number: FDSC0042

Animal: human

Sex and Age: F :44-year-old

Race: Japanese

Scientific Name: Homo sapiens

Tissue prepared: thyroid

Medium: Dulbecco's modified Eagle's medium with 10% fetal calf serum.

Passage method: Cells are treated with 0.02% EDTA and 0.25% trypsin

CO₂ Concentration: 5 %

Cell no. at passage: 1-5x10^5 cells/10cm dish

Genetics: thyroid carcinoma, female, 44-year-old

Life span: infinite

Characteristics: thyroid carcinoma, female, 44-year-old

Cell ID data: available

DNA Profile (STR): D5S818:11,12
D13S317:10,12
D7S820:11,12
D16S539:9,12
vWA:14,15
TH01:6,7
Amelogenin:X
TPOX:8,9
CSF1PO:11,12

Virus DNA detected: CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] -- Description

Established by Inoue,D. & Sugawa,H.

Deposited by Enomoto,T.&Sugaya,H.

Special notice: Report to Enomoto, T. is required before using.

Cell bank: NIHS(JCRB)

References: (1)

Comments: The HTC/C3 is unique by STR-PCR (04/22/2001).

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), STR-PCR(3), Freezing process, Others(1), Others(2),

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 04202001: seed (JCRB0164) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

91.3
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB), NP, LDH examined. Human non HeLa..

Anchorage dependency:

Cell Identification:

RFLP data:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Intergen RB52405).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA at 37 C for 10 minutes. Subculture once every 5-6 days and change medium once in between the subculture.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

7.0 x 10^4 cells/ml

Additional comments:

This cell culture was devided into NIHS and JCRB becuase no Mycoplasmas contamination observed.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

STR-PCR(3),

Freezing process,

(Date downloaded: 2008-06-16)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Enomoto,T., Sugawa,H., Inoue,D., Miyamoto,M., Kosugi,S., Takahashi,T., Kitamura,N., Yamamoto,I., Konishi,J., Mori,T., and et-al Establishment of a human undifferentiated thyroid cancer cell line producing several growth factors and cytokines, Cancer, 65: 1971-1979, 1990.