Profile:Well differentiated human hepato cellular carcinoma cell line. |
(* The format of the table was revised on Apr.25.2003.)
Additional Information(1) Additional Information(2) |
[ Lot No.: 071585: token (JCRB0403) ] |
Tests for mycoplasma, bacteria, and fungi were negative. [ Culture condition of this lot.]
Passage method: 0.1% trypsin + 0.005% EDTA Growth temperature: 37 Cell No. at passage: 2.5x10^4/cm^2 Additional comments: Grow in serum free medium. High amount of alpha feto protein is produced. [ Additional Data ]
(Date downloaded: 2007-12-13) |
[ Lot No.: 091986: token (JCRB0403) ] |
Date cell culture started:09/19/86 Concentration of cells in an ampule: 1.4x10^7 cell/ml Viability under microscope (%): 91% Antibiotics used :* Sterility test has not completed yet. [ Culture condition of this lot.]
Passage method: 0.1% trypsin + 0.005% EDTA Growth temperature: 37 Cell No. at passage: 2.5x10^4/cm^2 [ Additional Data ]
(Date downloaded: 2007-12-13) |
[ Lot No.: 102287: deposit (JCRB0403) ] |
[ Culture condition of this lot.] [ Additional Data ]
(Date downloaded: 2007-12-13) |
[ Lot No.: 021296: seed (JCRB0403) ] |
Passage Number, P44 Cell culture was started without cloning from the previous stock. Date cell culture started:01/26/96 Concentration of cells in an ampule: 3.9x10^6 cell/ml Viability under microscope (%): 80.2 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Kariology: mode = 60 Chromosome frequency distribution: <56/11 57/6 58/3 59/6 60/11 61/7 62/3 >63/4 (51) Anchorage dependency: Yes Cell Identification: available [ Culture condition of this lot.]
Freezing medium: Culture medium with 5% DMSO. Passage method: Rinsed with PBS(-) and treated with 0.1% trypsin and 0.005% EDTA at 37 C. Growth temperature: 37 C Cell No. at passage: 2.5x10^4 cells/ml Memo: from lot.102287 by Okado,K.(Myco. negative by PCR) [ Additional Data ]
Additional Information (Date downloaded: 2007-12-13) |
[ Lot No.: 021396: distribution (JCRB0403) ] |
Passage Number, P44 Cell culture was started without cloning from the previous stock. Date cell culture started:01/26/96 Concentration of cells in an ampule: 3.9x10^6 cell/ml Viability under microscope (%): 80.2 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: Yes [ Culture condition of this lot.]
Passage method: Cells rinsed with PBS(-) and treated with 0.1% trypsin and 0.005% EDTA at 37 C. Growth temperature: 37 C Cell No. at passage: 2.5x10^4 cells/ml Memo: from lot.102287 by Okado,K. [ Additional Data ]
(Date downloaded: 2007-12-13) |
[ Lot No.: 040396: examination (JCRB0403) ] |
Passage Number, P50 Cell culture was started without cloning from the previous stock. Date cell culture started:03/04/96 Concentration of cells in an ampule: 3.3x10^6 cell/ml Viability under microscope (%): 75.2 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: Yes Cell Identification: available [ Culture condition of this lot.]
Freezing medium: 5% DMSO with culture medium. Passage method: Rinsed cells with PBS(-) and treated with 0.1%-trypsin & 0.005% EDTA at room temp. Growth temperature: 37 C Cell No. at passage: 2.5-4x10^4 cells/sq.cm. Memo: Status of this lot is changed to distribution because the seed lot was created duplicatedly. (10/20/2000 Mizusawa,H.). Additional comments: Cells originally proliferated by Okado,K.and later viability and mycoplasmas tested again on 10/02/2000 by Sasaki,A. from a frozen ampoule. [ Additional Data ]
Additional Information (Date downloaded: 2007-12-13) |
[ Lot No.: 102296: distribution (JCRB0403) ] |
Passage Number, P49 Cell culture was started without cloning from the previous stock. Date cell culture started:09/19/96 Concentration of cells in an ampule: 1.7x10^6 cell/ml Viability under microscope (%): 91.0 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, G6PD(type B), LDH, NP. (human). Anchorage dependency: Yes [ Culture condition of this lot.]
Passage method: Wash cells with PBS(-) once and treat cells with 0.1% trypsin and 0.02% EDTA for 5 min. at 37 C. Growth temperature: 37 C Cell No. at passage: 2.2 x 10^5 cells/ml Memo: from lot.021296 by Nishimura,K. [ Additional Data ]
(Date downloaded: 2007-12-13) |