JCRB0136.1 CHO(pMAM-luc)
細胞情報
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0136.1 | 細胞名 | CHO(pMAM-luc) |
|---|---|---|---|
| 生物種(日本語) | チャイニーズハムスター | 組織名(日本語) | 卵巣 |
| コメント(日本語) | CHO-K1由来, ルシフェラーゼ遺伝子を含むpMAM-luc導入株 | プロフィール | pMAM-luc transfected CHO-K1, luciferase activated by dexamethasone |
| 別名 | 動物名 | hamster, Chinese | |
| 系統名 | Chinese | 学名・属名 | Cricetulus |
| 種名 | griseus | 性別 | |
| 年齢・月齢 | 細胞識別情報 | ||
| （癌）原発組織名 | ovary | 病歴情報 | genetically engineered CHO-K1 cells. External gene fragment(s) are introduced. |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | Plasmid pMAM-luc transfected CHO-K1 cells. Luciferase activated by dexamethasone(2uM) at 37C, 5 hrs. | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | ||
| 一般性状 | Luciferase gene under control of MMTV-LTR in plasmid. | 細胞分類 | transformed |
| 細胞樹立者名 | Mizuno,S. et al. | 細胞寄託者 | Mizuno,S. |
| 分譲時制限 | not identified | コメント | See CHO-K1 cell culture methods. |
| 入手年 | 1998 | 培養培地 | Ham's F12 medium with 10% fetal bovine serum. |
| 継代方法 | Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. | 継代時細胞数 | split ratio=1/10 |
| 人種 | 炭酸ガス濃度 | 5 % | |
| 採取組織名 | ovary | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
not tested | ウイルスRNA 検出検査 |
not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9538196 | Suppression of thermotolerance development through cycloheximide-induced negative control of stress protein gene expression. Akagawa H,Ishii A,Mizuno S J Biochem. 1998 Feb;123(2):226-32 |
| Pubmed id:9113384 | Stress dose-dependent suppression of heat shock protein gene expression by inhibiting protein synthesis during heat shock treatment. Mizuno S,Ishii A,Murakami Y,Akagawa H Cell Struct Funct. 1997 Feb;22(1):7-13 |
| Pubmed id:8689565 | Heat shock transcription factors: structure and regulation. Wu C Annu Rev Cell Dev Biol. 1995;11():441-69 |
| Pubmed id:8355691 | The DNA-binding activity of the human heat shock transcription factor is regulated in vivo by hsp70. Mosser DD,Duchaine J,Massie B Mol Cell Biol. 1993 Sep;13(9):5427-38 |
| Pubmed id:3422754 | Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals. Rodriguez JF,Rodriguez D,Rodriguez JR,McGowan EB,Esteban M Proc Natl Acad Sci U S A. 1988 Mar;85(5):1667-71 |
| Pubmed id:3821727 | Firefly luciferase gene: structure and expression in mammalian cells. de Wet JR,Wood KV,DeLuca M,Helinski DR,Subramani S Mol Cell Biol. 1987 Feb;7(2):725-37 |
| Pubmed id:17758108 | Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Ow DW,DE Wet JR,Helinski DR,Howell SH,Wood KV,Deluca M Science. 1986 Nov 14;234(4778):856-9 |
| Pubmed id:3927293 | Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment. Voellmy R,Ahmed A,Schiller P,Bromley P,Rungger D Proc Natl Acad Sci U S A. 1985 Aug;82(15):4949-53 |
| Pubmed id:5271752 | Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Kao F,Chasin L,Puck TT Proc Natl Acad Sci U S A. 1969 Dec;64(4):1284-91 |
| Images |
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LOT Information
| 細胞番号 | JCRB0136.1 | 細胞名 | |
|---|---|---|---|
| 培養ロット番号 | 092999 | 培養種別 | distribution |
| 培地 | Ham's F12 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 1-2 x 10^5 cells/ml | 継代方法 | 0.25% trypsin and 0.02% EDTA |
| 増殖速度 | D.T.=ca 24 hrs. | 凍結時生細胞濃度 | 2.2 x 10^6 |
| 凍結時生細胞率 | 98 | 使用抗生物質 | free |
| 継代数 | P8* | PDL数（プライマリ） | |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | Confirmed as Chinese hamster by NP, G6PD, MD. |
| 染色体モード | 染色体情報 | ||
| 表面抗原 | DNA Profile (STR) | ||
| 接着性 | 導入外部遺伝子 | ||
| 凍結培地 | 炭酸ガス濃度 | ||
| 解凍後生細胞率 | 追加情報 |