JCRB0153.3 tsJT60
細胞情報
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0153.3 | 細胞名 | tsJT60 |
|---|---|---|---|
| 生物種(日本語) | ラット | 組織名(日本語) | 胎仔 |
| コメント(日本語) | 3Y1由来, 温度感受性変異株, G0期で細胞周期が停止, (Fischer rat) | プロフィール | Rat 3Y1 derived ts mutant cell line with cell cycle arrest at G0 phase. |
| 別名 | tsJT60(3Y1) | 動物名 | rat |
| 系統名 | Fischer 344 | 学名・属名 | Rattus |
| 種名 | norvegicus | 性別 | F |
| 年齢・月齢 | fetus | 細胞識別情報 | |
| （癌）原発組織名 | embryo | 病歴情報 | normal |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | A ts mutant for cell cycle progression from G0 to S phase. | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | epithelial-like | |
| 一般性状 | 3Y1 derived ts mutant cell line with MNNG treatment. Cells grow at 34 C and are arrested at G0 at 40 C. Cells continue proliferation at both temperatures in cell cycle. | 細胞分類 | mutant |
| 細胞樹立者名 | Ninomiya-Tsuji,J. and Ide,T. | 細胞寄託者 | Kyoizumi,S. |
| 分譲時制限 | free | コメント | |
| 入手年 | 2000 | 培養培地 | Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal calf serum. |
| 継代方法 | Cells harvested after treatment with 0.125% trypsin and 0.01% EDTA. Subculture once a week at split ratio=1/8 at 34 C. | 継代時細胞数 | 5-8 x 10^5 cells/60 mm dish |
| 人種 | 炭酸ガス濃度 | 5 % | |
| 採取組織名 | embryo | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
not tested | ウイルスRNA 検出検査 |
not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7698232 | Induction of gadd153 gene in G0-specific ts mutant cells at nonpermissive temperature. Kashima N,Ueohzono T,Fornace AJ Jr,Ide T Exp Cell Res. 1995 Apr;217(2):324-8 |
| Pubmed id:2150790 | Nonlethal G0-ts mutant tsJT60 becomes lethal at the nonpermissive temperature after transformation: a hint for new cancer chemotherapeutics. Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T Cell Struct Funct. 1990 Dec;15(6):385-91 |
| Pubmed id:3169150 | A cell cycle G0-ts mutant, tsJT60, becomes lethal at the nonpermissive temperature after transformation with adenovirus 12 E1B 19K mutant. Tai YY,Goto Y,Ninomiya-Tsuji J,Kameoka Y,Ishibashi S,Shiroki K,Ide T Exp Cell Res. 1988 Nov;179(1):50-7 |
| Pubmed id:2822238 | Entrance of SV40-transformed cells into G0 phase as revealed by a study using the G0-specific ts mutant tsJT60. Ninomiya-Tsuji J,Ishibashi S,Ide T Cancer Res. 1987 Nov 15;47(22):6028-32 |
| Pubmed id:3499990 | Bypass of the ts block of tsJT60, a G0-specific ts mutant from rat fibroblasts, by fetal bovine serum and epidermal growth factor. Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T Cell Struct Funct. 1987 Oct;12(5):421-32 |
| Pubmed id:3040451 | Induction of cellular DNA synthesis in G0-specific ts mutant, tsJT60, following infection with SV40 and adenoviruses. Ninomiya-Tsuji J,Goto Y,Ishibashi S,Shiroki K,Ide T Exp Cell Res. 1987 Aug;171(2):509-12 |
| Pubmed id:3497814 | Epidermal growth factor has a unique effect in combination with fetal bovine serum to bypass the ts-block of G0-specific ts mutant tsJT60. Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T Exp Cell Res. 1987 Jul;171(1):86-93 |
| Pubmed id:3801408 | Expression of growth-regulated genes in tsJT60 cells, a temperature-sensitive mutant of the cell cycle. Ide T,Ninomiya-Tsuji J,Ferrari S,Philiponis V,Baserga R Biochemistry. 1986 Nov 4;25(22):7041-6 |
| Pubmed id:2423350 | Defect in prereplicative phase of G0-specific ts mutant, tsJT60. Ninomiya-Tsuji J,Goto Y,Ishibashi S,Ide T Exp Cell Res. 1986 Jul;165(1):191-8 |
| Pubmed id:6692850 | Isolation of a G0-specific ts mutant from a Fischer rat cell line, 3Y1. Ide T,Ninomiya J,Ishibashi S Exp Cell Res. 1984 Jan;150(1):60-7 |
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LOT Information
| 細胞番号 | JCRB0153.3 | 細胞名 | tsJT60 |
|---|---|---|---|
| 培養ロット番号 | 02152001 | 培養種別 | distribution |
| 培地 | Dulbecco's modified Eagle's medium with 10% fetal calf serum (Mitsubishi PFCS PVF001). | 培養温度 | 37 C |
| 継代時細胞数（濃度） | 7.5 x 10^3 cells/sq.cm. | 継代方法 | Cells are harvested after treatment with 0.125% trypsin and 0.01% EDTA. |
| 増殖速度 | NT | 凍結時生細胞濃度 | 1.7 x 10^6 |
| 凍結時生細胞率 | 90.6 | 使用抗生物質 | free |
| 継代数 | P10* | PDL数（プライマリ） | NT |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | G6PD, NP, LDH examined. (rat). |
| 染色体モード | NT | 染色体情報 | NT |
| 表面抗原 | NT | DNA Profile (STR) | |
| 接着性 | Yes | 導入外部遺伝子 | NT |
| 凍結培地 | Culture medium with 5% DMSO. | 炭酸ガス濃度 | 5 % |
| 解凍後生細胞率 | 追加情報 |
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