JCRB0169 Lu-134-B
細胞情報
Important Notice(s)Culture medium for JCRB0169 Lu-134-B
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0169 | 細胞名 | Lu-134-B |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 肺(がん) |
| コメント(日本語) | 小細胞がん, classic type | プロフィール | Small cell lung carcinoma cell line established through heterotransplanted tumor. |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | M |
| 年齢・月齢 | 80 | 細胞識別情報 | available |
| （癌）原発組織名 | lung (cancer) | 病歴情報 | small cell carcinoma arising in the periphery of right lower lobe of lung. |
| 転移の有無（Y/N） | （癌）転移組織名 | ||
| 遺伝的性質 | Activities of gamma-enolase and aromatic L-amino acid decarboxylase are high significantly. Hypertetraploid chromosome pattern. M=85, Td=85 hrs | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | Cluster formation. | |
| 一般性状 | Derived from small cell lung carcinoma, classic type. Cells are prepared from heterotransplantation of the tumor into athymic BALB/c mice. Dense cored granule (neurosecretory granule) observed unde EM. | 細胞分類 | tumor |
| 細胞樹立者名 | Terasaki,T. and Shimosato,Y. | 細胞寄託者 | Terasaki,T. |
| 分譲時制限 | コメント | ||
| 入手年 | 2001 | 培養培地 | Eagle's minimal essential medium supplemented with glutamine and 10% heat-inactivated fetal calf serum. |
| 継代方法 | Same volume of a fresh medium as the cell culture was added to the cell suspension and pipetting twice or three times. Take a portion of the diluted cell suspension is applied to further cell culture. | 継代時細胞数 | split ratio = 1/2. |
| 人種 | Japanse | 炭酸ガス濃度 | 5 % |
| 採取組織名 | lung | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2168867 | Selection of radioresistant cells by vitamin A deficiency in a small cell lung cancer cell line. Terasaki T,Shimosato Y,Wada M,Yokota J,Terada M Jpn J Cancer Res. 1990 Aug;81(8):780-5 |
| Pubmed id:2438038 | Reversible squamous cell characteristics induced by vitamin A deficiency in a small cell lung cancer cell line. Terasaki T,Shimosato Y,Nakajima T,Tsumuraya M,Ichinose H,Nagatsu T,Kato K Cancer Res. 1987 Jul 1;47(13):3533-7 |
| Pubmed id:6098504 | Interconversion of biological characteristics of small cell lung cancer depending on culture conditions. Terasaki T,Kameya T,Nakajima T,Tsumuraya M,Shimosato Y,Kato K,Ichinose H,Nagatsu T,Hasegawa T Gan. 1984 Dec;75(12):1089-99 |
| Images |
|---|
         |
LOT Information
| 細胞番号 | JCRB0169 | 細胞名 | |
|---|---|---|---|
| 培養ロット番号 | 06022003 | 培養種別 | distribution |
| 培地 | RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | 培養温度 | 37 C |
| 継代時細胞数（濃度） | uncountable due to aggregates. Split ratio 1:6-1:3 | 継代方法 | Dilution with pipetting large cell aggregates to small fragments. |
| 増殖速度 | 凍結時生細胞濃度 | 5 - 10 x 10^6 | |
| 凍結時生細胞率 | ca. 70 | 使用抗生物質 | free |
| 継代数 | > P31 | PDL数（プライマリ） | |
| ﾏｲｺﾌﾟﾗｽﾞﾏ検出 | - | 細菌汚染検出 | - |
| 真菌汚染検出 | - | ｱｲｿｻﾞｲﾑ検査・動物名 | Confirmed as human by NP, G6PD (type B), MD and LD. |
| 染色体モード | 染色体情報 | ||
| 表面抗原 | DNA Profile (STR) | ||
| 接着性 | No | 導入外部遺伝子 | |
| 凍結培地 | 10% DMSO, 20% FBS - RPMI1640 | 炭酸ガス濃度 | 5% |
| 解凍後生細胞率 | 追加情報 |