JCRB0144.2D LYM-1.P3D

細胞情報

細胞種類:一般細胞 (細胞分譲手数料はこちら)

細胞番号(JCRB)   JCRB0144.2D 細胞名   LYM-1.P3D
生物種(日本語)   ラット 組織名(日本語)   リンパ節
コメント(日本語)    プロフィール   Serum free FAR-2.F60 rat cell culture from lymph node near colon cancer.
別名    動物名   rat
系統名   JAR-2 学名・属名   Rattus
種名   norvegicus 性別   
年齢・月齢    細胞識別情報   
(癌)原発組織名    病歴情報   tissue obtained from lymph node near colon cancer of the JAR-2 rat given MNU(0.4mg/ml). The MNU was applied to a bowel directly of two-week-old rat and tumors were found three mo. later. The lymph node was cultured by roller tubes with DM170 and 2% FBS.
転移の有無(Y/N)    (癌)転移組織名   
遺伝的性質   Subclone of the LYM-1.P3. Morphologys and karyotypes were different from the LYM-1.P3B. Karyotype is lower diploid. 細胞寿命   infinite
クライシスPDL    形態   fibroblast-like
一般性状   Culture fruid of the LYM-1 strongly inhibited a growth of AH-7974 and other several cell lines. The inhibition was strongly suppressed by heating. 細胞分類   serum free
細胞樹立者名   Kurayama,H. and Takaoka,T. 細胞寄託者   Takaoka,T.
分譲時制限   free コメント   from LYM-1
入手年   2015 培養培地   DM201 medium with serum free condition.
継代方法   Cells harvested by gentle scraping. 継代時細胞数   1.3 x 10^4 cells/sq.cm.
人種    炭酸ガス濃度   
登録状況   registered 採取組織名   lymph node near colon cancer
ウィルス検査    検出ウィルス   
組織型   


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LOT Information

細胞番号   JCRB0144.2D 細胞名   LYM-1.P3D
培養ロット番号   04132016 培養種別   distribution
培地   DM201 medium(Research Institute for the Functional peptides).Lipid and protein free condition. 培養温度   37 C
継代時細胞数(濃度)   2.5-3.27x10^4 cells/sq.cm 継代方法   Cells were harvested with scraper.
増殖速度   NT 凍結時生細胞濃度   2.84x10^6
凍結直後生細胞率   95 使用抗生物質   free
継代数   p9* PDL数(プライマリ)   NT
マイコプラズマ検出   - 細菌汚染検出   -
真菌汚染検出   - アイソザイム検査・動物名   NT
染色体モード   NT 染色体情報   NT
表面抗原   NT DNA Profile (STR)   
接着性   Yes 導入外部遺伝子   NT
凍結培地   FM-1 and DM201 medium(1/1) 炭酸ガス濃度   5%
RFPL所見    追加情報   


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