JCRB0151 T-Ag-MOSE

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0151 Cell Name   T-Ag-MOSE
Profile   Cells are derived from normal ovary of adult C3H/He mouse and immortalized by the plasmid pEF321-T containing T antigen gene. Cells are tumorigenic in nude mice. Other Name   
Animal   mouse Strain   C3H/He
Genus   Mus Species   musclus
Sex   F Age   8-weeks
Identity    Tissue for Primary Cancer   ovary
Case history   normal ovary of adult C3H/He mouse Metastasis   
Tissue Metastasized    Genetics   pEF321-T plasmid introduced cell line, laminin positive.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like, cobble-stone like. Character   immortalized by T antigen containing plasmid pEF321-T, tumorigenic in nude mice
Classify   transformed Established by   Kido,M. & Shibuya,M.
Registered by   Kido,M. Regulation for Distribution   not applied
Comment   5% CO2, 37 C culture. Original ampules were frozen with medium and 10% DMSO. Cells adheded less than 100%. Anchorage dependency is No. Original description. Year   1999
Medium   Dulbecco's modified Eagle's medium 10% fetal bovine serum. Methods for Passages   0.25% trypsin treatment when cells are harvested.
Cell Number on Passage   1.5 x 10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_C562
Reference
Pubmed id:9820870Isolation and characterization of mouse ovarian surface epithelial cell lines.
Kido M,Shibuya M
Pathol Res Pract. 1998;194(10):725-30

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LOT Information

Cell No.   JCRB0151 Cell Name   T-Ag-MOSE
LOT No.   07142000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Mitsubishi PFCS PVF001). Temperature   37 C
Cell Density at Seeding   1.7 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Medium change every 1-2 days. Subculture every 4-5 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   85.8 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP and LDH examined.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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