JCRB0152.1 M
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0152.1 | Cell Name | M |
|---|---|---|---|
| Profile | Cell line which is derived from RLC-3 (JAR rat) after 54 day-culture by Nagisa method with treatment of DAB(10ug/ml) for one week. Cells are capable to form collagen network. | Other Name | |
| Animal | rat | Strain | JAR |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | 10 days | |
| Identity | Tissue for Primary Cancer | liver | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | 4-dimethylaminoazobenzene(DAB) transformed and remarkable changes were found in the biochemical properties. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Morphological changes observed cell line by DAB treatment. Historical cell line produced by Nagisa culture and DAB treatment. |
| Classify | transformed | Established by | Katsuta,H. and Takaoka,T. |
| Registered by | Takaoka,T. | Regulation for Distribution | free |
| Comment | Reference cell line for the M.P3(NIHS0228.2 JCRB0152.2 under preparation). | Year | 2000 |
| Medium | DM160-AU medium with 2% fetal bovine serum. Add 10% fetal bovine serum for initial cell attachment after thawing. | Methods for Passages | Cells prepared after treatment with 0.25% trypsin. |
| Cell Number on Passage | Split ratio = 1/8. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
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| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7441906 | An attempt in tissue culture at preventing and treating the collagen fiber formation of liver cells. Katsuta H,Takaoka T Jpn J Exp Med. 1980 Aug;50(4):275-82 |
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LOT Information
| Cell No. | JCRB0152.1 | Cell Name | M |
|---|---|---|---|
| LOT No. | 08142000 | Lot Specification | distribution |
| Medium | DM201 medium with 2% fetal calf serum (Mitsubishi, PFCS PVF001). | Temperature | 37 C |
| Cell Density at Seeding | 1-4 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested with scraper and resuspend in fresh medium. Change medium every 2-3 days and subculture once a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 |
| Viability at cell freezing (%) | 94.3 | Antibiotics Used | free |
| Passage Number | P6* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, NP, LDH examined. Rat. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO and 10% fetal calf serum. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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