IFO50277 5RP7

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50277 Cell Name   5RP7
Profile   fibroblast transformed by c-Ha-ras oncogene Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast transformed by c-Ha-ras oncogene
Classify   transformed Established by   Garbsa, S. et al.
Registered by   Kanamaru,T. Regulation for Distribution   
Comment    Year   1990
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5490
Reference
Pubmed id:3028610Secretion of type IV collagenolytic protease and metastatic phenotype: induction by transfection with c-Ha-ras but not c-Ha-ras plus Ad2-E1a.
Garbisa S,Pozzatti R,Muschel RJ,Saffiotti U,Ballin M,Goldfarb RH,Khoury G,Liotta LA
Cancer Res. 1987 Mar 15;47(6):1523-8

Images



LOT Information

Cell No.   IFO50277 Cell Name   5RP7
LOT No.   1067 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free (previously treated with MC210, BM1, BM2)
Passage Number   Unknown (13 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
CLOSE